四神丸对溃疡性结肠炎模型大鼠Toll样受体2白细胞介素―1β和白细胞介素―4影响.docVIP

四神丸对溃疡性结肠炎模型大鼠Toll样受体2白细胞介素―1β和白细胞介素―4影响 　　摘要：目的 观察四神丸对溃疡性结肠炎（UC）模型大鼠结肠组织Toll样受体2（TLR2）基因表达及血清白细胞介素（IL）-1β、IL-4含量的影响，探讨其治疗UC的作用机制。方法 采用复合方法制作脾肾阳虚型UC大鼠模型。实验大鼠分为空白组、模型组、阳性药组、四神丸组，阳性药组予柳氮磺吡啶肠溶片水溶液0.5 g/kg灌胃，四神丸组予四神丸26 g/kg灌胃，每日1次，共21 d。肉眼观察结肠组织损伤情况，并检测大鼠结肠组织TLR2基因表达及血清IL-1β、IL-4的含量。结果 与空白组比较，模型组大鼠结肠黏膜肉眼观察有显著炎症、充血、溃疡形成（P0.01），模型组大鼠结肠组织TLR2基因表达与血清IL-1β含量升高（P0.05），IL-4含量降低（P0.01）；与模型组比较，四神丸组大鼠结肠组织TLR2基因表达和血清IL-1β含量均降低（P0.05，P0.01），IL-4含量升高（P0.05）。结论 四神丸治疗UC机制可能与调节肠道免疫细胞因子、抑制TLR2信号避免其过度激活有关。 　　关键词：四神丸；溃疡性结肠炎；Toll样受体2；白细胞介素-1β；白细胞介素-4；大鼠 　　DOI：10.3969/j.issn.1005-5304.2015.12.016 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）12-0064-04 　　Effects of Sishen Pill on Expressions of TLR2， IL-1β and IL-4 in Ulcerative Colitis Model Rats WANG Di， ZHU Xiang-dong， LI Lan-zhen， WANG Yan， HE Lan-juan， GUO Ting-ting （Gansu University of Chinese Medicine， Lanzhou 730000， China） 　　Abstract：Objective To observe the effects of Sishen Pill on expressions of TLR2， IL-1β and IL-4 in ulcerative colitis model rats；To investigate the mechanism of Sishen Pill for the treatment of ulcerative colitis. Methods Compound method was used to establish spleen-kidney-yang-deficiency ulcerative colitis model rats. Experimental rats were divided into blank group， model group， positive medicine group， and Sishen Pill group. Positive medicine group was given SASP aqueous solution 0.5 g/kg for gavage， while Sishen Pill group was given Sishen Pill 26 g/kg for gavage. The damage in colon tissue was observed with naked eyes. The expressions of TLR2 in colon tissue and IL-1β and IL-4 in serum were detected. Results Compared with the blank group， rats in the model group showed formation of inflammation， congestion and ulcer under general state and colonic mucosa visual inspection （P0.01）；TLR2 in colon tissue and IL-1β increased in serum （P0.05）；IL-4 level decreased （P0.01）. Compared with model group， TLR2 in colon tissue and IL-1β inseerum decreased significantly in the Sishen Pill group （P0.05， P0.01）；IL-4 lev