四种食源性肽改善光老化大鼠皮肤皱纹及屏障功能实验研究.docVIP

四种食源性肽改善光老化大鼠皮肤皱纹及屏障功能实验研究 　　[摘要]目的：?u价四种食源性肽口服暴露对SD大鼠光老化皮肤皱纹及屏障功能的改善效果。方法：将112只健康成年雌性SD大鼠随机平均分为空白对照组、模型组、肽样品组，每种肽分别设定低、中、高剂量组，配制浓度为0.3、0.9、1.5g/L的含肽水溶液进行口服，每日自由饮用；空白组不施行紫外辐照，模型组及肽样品组均行UVA+UVB联合辐照，连续辐照18周后进行皮肤表观皱纹评分，检测皮肤表皮水分含量和油脂含量，评价4种食源性肽对光老化大鼠皮肤皱纹和屏障功能的改善效应。结果：与正常对照组相比，模型组大鼠皮肤皱纹感官质量显著降低，肽口服干预后可不同程度改善皮肤皱纹状况，且总体呈剂量-效应关系，不同活性肽之间改善效果差异较大，以样品2高剂量组效果最好；从屏障功能来看，模型组大鼠皮肤水分和油脂含量显著低于空白组（P0.05）；4种活性肽口服干预后光老化皮肤水油屏障功能恢复良好，样品2和核桃肽中、高剂量时可显著提升表皮水分和油脂含量（P0.05）。结论：样品2能显著减少光老化大鼠皮肤深大皱纹量，改善受损皮肤屏障功能，是口服抗皱活性肽产品开发的首选基料。 　　[关键词]活性肽；口服；SD大鼠；光老化；屏障功能 　　[中图分类号]R758.14 [文献标志码]A [文章编号]1008-6455（2017）09-0049-05 　　Comparative Analysis of Four Kinds of Food-derived Peptides with Oral Supplementation on the Wrinkling and Barrier Function Status in Photoaging 　　Skin of SD Rat 　　XU De-feng1，2，MA Zhong-hua1，ZHAO Mou-ming2，ZHANG Ting1，SU Guo-wan2，DUAN Jun-yi3 　　[1.Technique Center of Infinitus （China） Co. Ltd.，Guangzhou 510665，Guangdong，China； 2.College of Light Industry And Food Science Technology，South China University of Technology，Guangzhou 510640，Guangdong，China； 3.College of Agriculture and Animal Husbandry，Tibet University， Linzhi 86000， Tibet，China] 　　Abstract： Objective To evaluate the improvement of four kinds of food-derived peptides with oral supplementation on wrinkling and barrier function status of SD rat photoaging. Methods 112 of healthy adult female SD rats were randomly divided into normal control group， ultraviolet exposed group and peptides administration groups. Except the normal group， all animals in model and peptides administration groups were exposed to radiation of UVA in companying with UVB. The low， middle and high-dose bioactive peptides administration were obtained by preparing the water solution of peptides with concentration of 0.3， 0.9 and 1.5g/L respectively. And then the obtained peptides solution was orally administrated to the SD rats in peptides treating groups as the sole drinking source. The model and peptides groups were exposed to UV irradiation thri