商洛不同产地连翘有效成分含量与土壤因子相关性研究.docVIP

商洛不同产地连翘有效成分含量与土壤因子相关性研究 　　摘 要：采用HPLC法测定了商洛五县区14个样方点的连翘药材中连翘苷、连翘酯苷A含量，采用理化分析法进行土壤因子的测定，并研究了连翘有效成分含量与土壤因子的关系。结果显示：有机质、速效钾与连翘苷的含量呈极显著正相关，速效钾与连翘酯苷A的含量呈显著正相关。土壤中丰富的有机质、速效钾供应会促进连翘药材体内连翘苷的形成和积累，但过多的有机质和碱解氮不利于连翘酯苷A的积累。近碱性（平均pH值为7.236）土壤条件会促进连翘药材中连翘苷的积累而抑制连翘酯苷A的积累。因此，无论是选择适宜产地或是实施科学施肥，都应重视和考虑有机质、速效钾、碱解氮及土壤pH值对连翘药材质量的影响。 　　关键词：连翘苷；连翘酯苷A；HPLC；土壤因子；相关分析 　　中图分类号：S567.1+9文献标识号：A文章编号：1001-4942（2016）05-0055-04 　　Abstract The contents of forsythin and forsythiaside A in Fructus Forsythiae from 14 sampling points of five counties in Shangluo were tested using HPLC， additionally， the edaphic factors were analyzed by physical and chemical analysis method to get the relationship between the active ingredient contents of Fructus Forsythiae and edaphic factors. The results indicated that the content of forsythin showed highly significant correlations with organic matter and available potassium， and the content of forsythiaside A was significantly correlated with available potassium. The abundant organic matter and available potassium contents in the soil could promote the formation and accumulation of forsythin in Fructus Forsythiae， while redundant organic matter and available nitrogen contents were not good for the accumulation of forsythiaside A. The nearly alkaline soil condition with the average pH value of 7.236 could promote the accumulation of forsythin but prevent that of forsythiaside A. Therefore， whether selecting suitable habitat or scientific fertilization， we all should pay attentions to the effects of organic matter， available potassium， available nitrogen and soil pH value on the quality of Fructus Forsythiae. 　　Key words Forsythin； Forsythoside A； HPLC； Soil factors； Correlation analysis 　　连翘为木犀科连翘属植物连翘[Forsythia suspensa （Thunb.） Vahl]的干燥果实，因其采收季节不同分为青翘和老翘。青翘为秋季果实初熟呈绿色时采收所得，老翘为秋季果实完全成熟后采收而得。连翘为中医最常用的中药材之一，历来被视为疮家要药[1]，性味苦，微寒，归心、肺、脾[2]，具有清热解毒、散结消肿之功效，用于风热感冒、温病初起、温热入营、热淋尿闭[3]、丹毒、斑疹等的治疗[4，5]。连翘有效成分包括：连翘酚、黄酮类、三萜类、连翘酯苷、连翘苷、挥发油类、熊果酸等[6，7]。已有研究发现，不同产地连翘中有效成分含量存在较为明显的差异