吸入氢气对脓毒症大鼠心肌损伤影响.docVIP

吸入氢气对脓毒症大鼠心肌损伤影响 　　【摘要】 目的：探讨吸入氢气对脓毒症大鼠心肌损伤的影响。方法：48只成熟雄性SD大鼠用随机数字表法分为4组，每组各12只：假手术组（SH组）、脓毒症组（CLP组）、1%氢气组（1%H2组），2%氢气组（2%H2组）。用盲肠结扎并穿刺法制作脓毒症大鼠模型，SH组不结扎穿刺盲肠，其他操作与CLP组相同，H2组在术后特定时点吸入相应浓度的氢气。造模后24 h采集血液后处死大鼠并分离左心室，检测血清中各组的心肌肌钙蛋白I（cTnI）和B型利钠肽（BNP）的水平以及心肌组织中内皮素-1（ET-1）、一氧化氮（NO）、瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）的表达水平，心肌用HE进行染色，观察心肌组织计算病理积分情况。结果：与SH组相比，其他各组的cTnI、BNP、ET-1、TNF-α、IL-6表达水平以及心肌病理评分均显著升高，NO水平降低，差异均有统计学意义（P0.05）。结论：吸入氢气可以改善脓毒症大鼠的心肌损伤，其可能机制与改善心肌组织微循环和抑制炎症反应有关。 　　【关键词】 氢； 脓毒症； 心肌损伤 　　【Abstract】 Objective：To investigate the effect of hydrogen inhalation on sepsis induced myocardial injury in rats.Method：48 Sprague-Dawley male rats were randomly divided into 4 groups with a random number table，12 cases in each group：sham operation group（SH group），CLP group，1%H2 group and 2%H2 group.The rats model of sepsis were induced by ligation and puncture of the appendix， and the SH group was not ligation and puncture of the appendix， the other operation was the same as that in the CLP group， and the H2 groups at the particular moment after the operation of the corresponding concentration of hydrogen.The rats were killed after collecting blood at modeling for 24 h and the left ventricular was separated，the levels of serum cardiac troponin I （cTnI） ，B-type natriuretic peptide （BNP）， myocardial tissue endothelin-1 （ET-1）， nitric oxide （no）， tumor necrosis factor alpha （TNF alpha） and leukocyte mediated IL-6 （IL-6） expression of all groups were detected，hematoxylin and eosin （he） staining was given to stain the myocardial，then the myocardial tissue pathological integral calculation was observed. Result：Compared with SH group，the myocardial pathological scores and levels of cTnI，BNP， ET-1， TNF-α and IL-6 were significantly increased in CLP，1%H2 and 2%H2 groups，while the level of NO was significantly decreased in CLP，1%H2 and 2%H2 groups，the differences were statistically significant（P0.05）.Conclusion：Inhalation of hydrogen can alleviate sepsis induced myocardial injury in rats， the me