基于ISSR标记油茶种质资源遗传基础分析.docVIP

基于ISSR标记油茶种质资源遗传基础分析 　　摘要：以油茶（Camellia oleifera Abel.）主产区的109个种质材料为试材，采用简单序列重复区间分子标记（ISSR）和非加权组平均法（Unweighted pair-group method with arithmetic means，UPGMA）聚类分析，研究了这109个油茶种质材料遗传相似系数及亲缘关系分析，并构建了其进化树关系。结果表明，①从49条ISSR引物中筛选出23条能够扩增出清晰，稳定条带的引物，23条引物共扩增出487条带，其中多态性条带有335条，多态性条带比率为68.79%；②应用NTSYS2.10e软件对扩增结果进行UPGMA法聚类分析，109个油茶种质材料的遗传相似系数从0.61到0.93；③油茶种质资源丰富，遗传相似系数在0.735时，可将109个油茶种质材料划分为11个类群。类群I包括79个种质材料，该类群在相似系数为0.75处又可划分为7个亚类群，且这些油茶种质材料间的亲缘关系较近。但由聚类分析划分的油茶类群来看，这些油茶材料的遗传背景又比较复杂，具有丰富的遗传多样性。分析结果可为油茶主产区的品种资源开发、基因资源保存、后期品种审定及鉴别提供理论依据。 　　关键词：油茶（Camellia oleifera Abel.）；种质资源；简单序列重复区间标记；遗传多样性 　　中图分类号：S794.4+95：Q943.2 文献标识码：A 文章编号：0439-8114（2018）02-0119-07 　　DOI：10.14088/j.cnki.issn0439-8114.2018.02.030 　　Abstract： 109 germplasm materials of Camellia oleifera Abel in the main producing areas were analyzed using inter simple sequence repeat markers（ISSR） and Unweighted pair-group method with arithmetic means （UPGMA） cluster analysis method to study the genetic correlation coefficient and genetic relationship， and constructe the phylogenetic relationship. The result showed that， （1） 23 primers with clear and stable bands were screened from 49 ISSR primers， and 487 bands were amplified by the 23 primers， of which 335 bands were polymorphic，and the polymorphic bands ratio was 68.79%.（2） NTSYS2.10e software was used to cluster the amplified results by UPGMA method； and the genetic similarity coefficients of 109 C. oleifera germplasm materials were from 0.61 to 0.93. （3） The germplasm resources of C. oleifera were rich. When the genetic similarity coefficient was 0.735， the 109 C. oleifera germplasm materials could be divided into 11 groups. The group I includes 79 germplasm materials， which can be divided into 7 sub groups at the similarity coefficient of 0.75， and the genetic relationship among these C. oleifera germplasm materials is closer. However， the genetic background of these C. oleifera germplasm materials is complex and rich in genetic diversity. The analysis results c