基于多糖和黄酮类成分铁皮石斛优良内生真菌筛选.docVIP

基于多糖和黄酮类成分铁皮石斛优良内生真菌筛选 　　[摘要] 为了揭示铁皮石斛内生真菌与功效成分的关系，该研究利用HPLC和紫外分光光度法测定21种铁皮石斛内生真菌菌丝及发酵液提取物中糖类和黄酮类成分。结果表明：21个供试菌株发酵产物乙酸乙酯提取物中均可检测到黄酮类成分，对比色谱保留时间和紫外光谱特征，发现DO49可产柚皮素，DO23，DO81，DO83可产芦丁；21个供试菌株发酵产物水提取物中均检测到多糖，但不同菌株单糖的组成存在差异，参照单糖种类以及甘露糖/葡萄糖的峰面积比，筛选与铁皮石斛多糖相关的优良菌株DO23，DO26，DO81，DO54，DO55，DO83。综合糖类和黄酮类两类成分，筛选出优良菌株DO23，DO81，DO83，既能产生与铁皮石斛相同的黄酮成分，又能产生单糖组分与铁皮石斛相近的多糖。 　　[关键词] 铁皮石斛； 内生真菌； 单糖组成； 黄酮成分； HPLC 　　Screening of meaningful endophytic fungi in Dendrobium officinale based on polysaccharides and flavonoids 　　YU Mengmeng1， WU Lingshang1*， SI Jinping1， LIU Jingjing1， YU Zhixiang1， WANG Huacheng1， ZHANG Min2， RONG Song3 　　（1Zhejiang Agricultural and Forestry University， Nurturing Station for State Key Laboratory 　　of Subtropical SilvcultureLinan 311300， China； 2Huagang Management Office of Landscape 　　Architecture and Administration of Cultural Heritage of Hangzhou， Hangzhou 310013， China； 　　3Zhejiang Transfar Agribio Co， Ltd， Hangzhou 310013， China） 　　[Abstract] To reveal the relationship between endophytic fungi and the functional components， saccharides and flavonoids in the mycelia or fermented liquor of 21 endophytic fungi in Dofficinale were detected using HPLC and UV spectrophotometerThe results showed that the ethyl acetate extracts from 21 fungal strains all contain flavonoidsAccording to the chromatographic retention time of HPLC and UV spectra characteristics of flavonoids， strain DO49 was found produce naringenin， strains DO23， DO81 and DO83 were found produce rutinThe watersoluble extracts from 21 strains all had polysaccharidesHowever， there was difference in the composition of monosaccharides derived from polysaccharides among different strainsAccording to the composition of monosaccharides and the peak area ratio of mannose and glucose， the fungal strains including DO23， DO26， DO81， DO54， DO55， DO83 product polysaccharides associated with Dofficinale were selectedIn conclusion， based on the saccharides and flavonoids， the excellent endophytic fungal s