第十四期博士生毕业论文摘要集.docx

上海交通大学博士生学术论坛第十四期暨『海洋与可持续发展』学术论坛 PAGE \* MERGEFORMAT53 PAGE \* MERGEFORMATIII 第十四期博士生学术论坛 论文摘要集 声明 本论文集收录的论文不作公开发表，只作为内部资料保存和交流，请勿对外传阅，违者后果自负。 目 录 PART Ⅰ 论文集………………1 PART Ⅱ 论文摘要集…………44 “数字与媒体”上海交通大学第二期博士生学术论坛策划 PAGE 18 Part I 论文集 目 录 01 Simple DNA Extraction Device and Its Combination with Modified Visual Loop-Mediated Isothermal Amplification for Rapid On-Field Detection of Genetically Modified Organisms………………………1 Miao Zhang 02 PDCD5基因在发育中的自噬重构作用…………………………13 安琳，朱洪新 03 An opportunistic pathogen isolated from the gut of an obese human causes obesity in germfree mice ……………………………19 Na Fei, Liping Zhao 04 Structural Changes of Gut Microbiota during Berberine-Mediated Prevention of Obesity and Insulin Resistance in High-Fat Diet-Fed Rats……………………………………………………………………25 Xu Zhang, Yufeng Zhao 上海交通大学博士生学术论坛第十四期暨『海洋与可持续发展』学术论坛 One Simple DNA Extraction Device and Its Combination with Modified Visual Loop-Mediated Isothermal Amplification for Rapid On-Field Detection of Genetically Modified Organisms Miao Zhang (Shanghai Jiao Tong Univesity, Shanghai 200240) Quickness, simplicity, and effectiveness are the three major criteria for establishing a good molecular diagnosis method in many fields. Herein we report a novel detection system for genetically modified organisms (GMOs), which can be utilized to perform both on-field quick screening and routine laboratory diagnosis. In this system, a newly designed inexpensive DNA extraction device was used in combination with a modifi ed visual loop-mediated isothermal amplification (vLAMP) assay. The main parts of the DNA extraction device included a silica gel membrane filtration column and a modified syringe. The DNA extraction device could be easily operated without using other laboratory instruments, making it applicable to an on-field GMO t est. High -quality gen o mi c D NA (gDNA) suit able for polymerase chain reaction (PCR) and isothermal amplification could be quickly isolated from plant tissues using this device wit