奥曲肽联合5氟尿嘧啶对Hepa16肝癌细胞PAK1表达影响.docVIP

奥曲肽联合5氟尿嘧啶对Hepa16肝癌细胞PAK1表达影响 　　[摘要]目的研究不同浓度奥曲肽(OCT)及OCT联合5-氟尿嘧啶(5-Fu)对Hepa1-6肝癌细胞的作用效果，并探讨其可能的作用机制。方法 采用MTT法检测不同浓度OCT及OCT联合5-Fu对细胞活性的影响；采用逆转录聚合酶链反应(RT-PCR)检测PAK1mRNA的表达；细胞划痕愈合实验测定细胞扩散运动能力。结果 不同浓度OCT及OCT联合5-Fu组细胞生长抑制率均随剂量的增加而增加，与对照组相比有显著差异(P＜0.01)，且联合用药组均明显高于相应浓度OCT及5-Fu组(P均0.05)；OCT10mg/L联合5-Fu组较肝癌细胞组、OCT10mg/L组PAK1mRNA的表达明显下调(P均0.01)，但仍较正常肝细胞组高；OCT10mg/L联合5-Fu组较肝癌细胞组、OCT10mg/L组细胞移行运动能力显著减弱。结论 OCT10mg/L联合5-Fu为抑瘤最佳药物浓度，其主要通过减少PAK1的表达、减弱细胞移行运动能力而发挥抑瘤作用。 　　[关键词]肝细胞癌；奥曲肽；5-氟尿嘧啶； PAK1 　　[中图分类号] R969.4[文献标识码]A [文章编号] 1005-0515（2010）-12-003-02 　　 　　The effect of combinating octreotide acetate and 5-Fluorouracil for the expression of PA 　　Ping Mei1,2Zhao He-ping2 　　(1.Shanxi Medical University,Taiyuan 030001;2.Department of Synthetical Section, 　　the First Affiliated Hospital of Shanxi Medical Uiversity,Taiyuan 030001） 　　[Abstract]Objective To research the effect of different concentration of Octreotide Acetate（OCT）and OCT combinate 5-Fluorouracil(5-FU) on hepatoma(Hepa1-6) cell,and to explore its role in the mechanism.Methods MTT method was used to test the activity of the cell acted by assay of different concentrations OCT and OCT combinate 5-Fu;the expression of PAK1mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR);monolayer wound healing assay was used to detect Hepa1-6 cell motility.Results The inhibitory rate of cell growth in different concentrations of OCT and OCT combined with 5-Fu group increased with OCT dosage augment,and was significantly higher than that in controls(P＜0.01),and the combined treatment group were significantly higher than the corresponding concentration of OCT group and 5-Fu group(P0.05);The relative level of PAK1mRNA in OCT 10mg/L combined with 5-Fu group was significantly decreased compared with hepatocellular carcinoma group and OCT 10mg/L group(P0.01),but still higher than normal group; the cell migration ability of OCT 10mg/L combined with 5-Fu group was weaken significantly compared with hepatocellular