大鼠肾缺血再灌注后p38MAPK蛋白表达变化及银杏达莫注射液对其影响.docVIP

大鼠肾缺血再灌注后p38MAPK蛋白表达变化及银杏达莫注射液对其影响 　　摘 要：目的：探讨银杏达莫注射液对肾缺血-再灌注损伤的保护作用及机制。方法：肾缺血1h再灌注1h建立大鼠肾缺血再灌注损伤模型，用银杏达莫注射液分别按剂量0.9、1.8、3.6mL/kg预处理7天。通过检测肾组织丙二醛含量、超氧化物歧化酶的活性及血肌酐、血尿素氮的含量。电镜下观察各组肾组织的形态学变化，Western印迹法观察p38 MAPK蛋白的活化情况。结果：缺血再灌注损伤后，肾组织出现明显病理改变，肾组织丙二醛含量升高，超氧化物歧化酶活性下降，血肌酐、尿素氮水平升高，肾组织p- p38MAPK蛋白水平显著上升。银杏达莫注射液预处理能降低血肌酐、尿素氮水平及肾组织丙二醛含量，增强超氧化物歧化酶活性和下调p- p38MAPK蛋白水平，与模型组比较有显著性差异（P0.05和P0.01）。电镜观察肾组织的病理改变也明显好转。银杏达莫注射液不同剂量组之间无明显差异。结论：银杏达莫注射液对肾缺血再灌注损伤有良好的保护作用，其机制可能是通过清除氧自由基、减轻脂质过氧化反应而下调p38MAPK蛋白的表达以减轻再灌注损伤程度，进而大鼠肾功能。 　　关键词：肾；缺血-再灌注损伤；p38MAPK；银杏达莫注射液 　　中图分类号：R692文献标识码：A 文章编号：1673-7717（2011）04-0856-05 　　Effect of Injection of Ginkgo Biloba Extract and Dipyridamole on 　　p38MAPK Activation in Rats with Renal Ischemia Reperfusion 　　LI Dan【sup】2【/sup】,ZHANG Yan-hua【sup】2【/sup】, CHEN Shao-jie【sup】2【/sup】,YU Xu-guang【sup】1 【/sup】，LIN Xiang-dong【sup】1 【/sup】， 　　TANG Lan-lan【sup】1【/sup】,WANG Shu-jun【sup】2【/sup】,WANG Yuan-yuan【sup】2【/sup】, LI Guan-long【sup】2【/sup】, WANG Wan-tie【sup】2【/sup】 　　（1. The affiliated Yueqing Hospital, Wenzhou Medical College，Wenzhou 325035，Zhejiang，China； 　　2. Department of Pathophysiology, Wenzhou Medical College，Wenzhou 325035，Zhejiang，China） 　　Abstract:Objective：To explore the mechanism of protective effects of Ginkgo biloba extract and Dipyridamole injection （XGD） on renal ischemia-reperfusion injury （RIRI）. Methods：The model of renal ischemia-reperfusion injury in male rats was made by removing the right renal ,and clamping of left renal artery for 1 hour and 1 hour of reperfusion.The rats with pretreatment were fed with XGD respectively at the dosage of 0.9,1.8,3.6mL/kg prior to operation for a week, The content of malondialdehyde （MDA）, the activity of superoxide dismutase （SOD） in renal tissue and the levels of serum creatinine （Scr）, blood urea nitrogen （BUN） were measured. The morphologic changes of renal tissue were observed by electronic microscope. western bloting was used to detect the protein expression and activation.Results：After ischem