大黄对重症急性胰腺炎影响及细菌移位研究.docVIP

大黄对重症急性胰腺炎影响及细菌移位研究 　　[摘要] 目的:采用牛磺胆酸钠诱发大鼠重症急性胰腺炎模型,探讨大黄对重症急性胰腺炎的影响及细菌移位。方法:健康Wistar大鼠30只,雌雄不限,随机分成三组。1组为SAP模型组(M组,n=10),开腹后肝端胰胆管用小动脉夹夹闭,肠端胰胆管用丝线结扎。5%牛磺胆酸钠溶液0.1 ml/100 g胰胆管内以0.2 ml/min速度逆行推注,复制SAP模型;2组为中药大黄治疗组(T组,n=10),按M组方法复制SAP模型,并在复制模型后即刻胃管内注入生大黄粉溶液1 ml(5 mg/100 g)。3组为假手术组(SO组,n=10),开腹后自胰管内逆行注入生理盐水0.1 ml/100 g后关腹。术后取血液、胰腺和肠系膜淋巴结进行细菌培养。结果:脏器及血细菌培养结果显示,SO组血培养阴性,除2只大鼠肠系膜淋巴结培养出细菌外,其他脏器培养均阴性。M组细菌培养阳性率较高(P0.01),T组细菌培养阳性率明显低于M组(P0.01)。结论:SAP时细菌移位的发生率较高,可能与机体的细胞免疫功能下降有关。大黄能抑制肠菌及清理肠道,从而降低胰腺感染率,对SAP有一定的治疗作用。 　　[关键词] 急性胰腺炎;细菌移位;细菌培养;大黄 　　[中图分类号] R657.5+1[文献标识码]A [文章编号]1673-7210(2010)01(c)-024-03 　　 　　Effect of Rhubarb on severe acute pancreatitis and study of bacterial translocation 　　JIANG Liangfu 　　(Department of the First Section of Pediatric Surgery, Linyi Peoples Hospital of Shandong Province, Linyi 276000, China) 　　[Abstract] Objective: To explore the effect of Rhubarb on severe acute pancreatitis and bacterial translocation by establishing the rat severe acute pancreatitis model with Sodium Taurocholate. Methods: 30 Wistar rats were randomly divided into three groups, model group of SAP (group M, n=10) was induced by injecting adversely 5% Sodium Taurocholate for 0.2 ml/min (0.1 ml/100 g) into the common biliary-pancreatic duct; and saline was used in the rats of sham operation group (group SO, n=10) instead of Sodium Taurocholate. Therapeutic group (group T, n=10) was made as same as group M besides 1 ml Rhubarb(50 mg/kg) was injected into rats stomach on the instant after operation. Bacterial culture in pancreas, blood and mesenteric lymph nodes were collected to culture the bacteria. Results: In group SO, blood culture showed negative result, and no organism was found in organs except mesenteric nodes in two rats. The bacterial positive rate of group M was high (P0.01), and it was higher than that of group T (P0.01). Conclusion: The bacterial translocation occurs in SAP shows a high incidence, it may be related to the lower cellular immune function. Rhubar