WNK1多态性与蒙古族原发性高血压相关性研究-内科学专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要······························································································· （1） 英文摘要······························································································· （3） 论文题目······························································································· （5） 材料与方法·························································································（8） 结果································································································ （11） 讨论································································································ （14） 结论································································································ （16） 参考文献·························································································· （16） 文献综述····························································································· （20） 参考文献·························································································· （29） 缩略语表····························································································· （35） 攻读学位期间发表文章情况····································································· （36） 个人简历····························································································· （37） 致谢··································································································· （38） 1 中 文 摘 要 目的 原发性高血压（essential hypertension，EH）是人类巨大的健康和经济负担，是 冠心病、脑中风等众多心血管疾病的最大的独立危险因素，是由遗传和环境因素共同作 用而引起的复杂性疾病，寻找原发性高血压的相关致病基因和危险因素对高血压的防治 具有重要意义。WNK1（with no lysine (K) kinase 1）基因是最新发现的一种类丝氨酸/ 苏氨酸蛋白激酶, 目前国内外诸多研究均显示 WNK1 基因多态性与普通人群动脉血压 的变化相关。而蒙古族是我国高血压病患病率最高的五个民族之一，所以本课题旨在以 内蒙古蒙古族群体为研究对象初步探讨 WNK1 基因多态性与蒙古族原发性高血压的相 关性及临床意义。 方法 采用聚合酶链式反应-限制性片段长度多态性方法，随机选取 2012-2014 年就诊于 内蒙古医科大学第一附属医院的 110 例蒙古族人（高血压患者 60 例，正常对照 50 例）, 对 WNK1 基因 4 个 tagSNPs 即 WNK1 基因 Exon1 第 258 位， Exon2 第 126 位，Exon4 第 134 位，Exon6 第 79 位的多态性位点进行检测，并应用统计分析研究 WNK1 基因 tagSNPs 与原发性高血压发生的相关性。 结