SSTR靶向超顺磁性氧化铁的制备及其显像的实验研究-影像医学与核医学专业论文.docxVIP

SSTR 靶向超顺磁性氧化铁的制备及其显像的实验研究 中文摘要 目的：探讨载带 oct 的超顺磁性氧化铁（oct-USPIO）的制备方法；研究 oct-USPIO 的体内分布；探讨 oct-USPIO 作为 SSTR 靶向肿瘤显像剂的可能性。 方法：使用共沉淀法制备 USPIO，耦联 oct，再标记上 99mTc，获得 99mTc-oct-USPIO， 用扫描电镜检测其粒径，用离心法测定其放射化学纯度；小鼠尾静脉注 射 oct-USPIO，分别于注射后不同时间取出肝脏、脾、肾等脏器或组织，消化后测 量其含铁量，计算每克脏器中含铁量占注入总铁量的百分数（ %ID/g）；尾静脉 注射 99mTc-oct-USPIO 于荷乳腺癌小鼠体内，不同时间点进行 SPECT 显像，评估 其作为乳腺癌及其它肿瘤 SPECT 显像剂的可能性。 结果：USPIO 粒径约 10nm 左右，大小分布均匀，其混悬液的铁的含量（7.96±1.23） mg/mL．oct-USPIO 粒径约 20nm，大小分布均匀；99mTc-oct-USPIO 放射化学纯 度为(93.47±1.28)%；小鼠静脉注射 oct-USPIO 主要分布的脏器为肝脏。血液在 1 分钟达到最高（68.55±8.33）%ID/g，随后迅速降低；oct-USPIO 在肝脏的分布 在 8h 达到高峰，为（58.34±12.12）%ID/g，肺脏在 1 min 时其%ID/g 值最高为 （18.89±4.67），随时间推移其值逐渐降低。其余组织脏器也有一定的放射性；静 脉注射 99mTc-oct-USPIO 后，乳腺癌 SPECT 靶与非靶比较高，但肝脏的靶与非靶 更高。 结论：Oct-USPIO 粒径约 20nm，分布均匀，性质稳定；99mTc-oct-USPIO 放射化 学纯度高，性质稳定；静脉注射 oct-USPIO 后，其主要分布于肝脏，血液清除快； 99mTc-oct-USPIO 荷乳腺癌小鼠 SPECT 显像瘤体显示较为清晰，有作为乳腺癌及 其它肿瘤 SPECT 和 MRI 显像剂的可能性。 关键词：oct-USPIO；制备；显像 The preparation and imaging experiment research of SSTR targeted Ultrasmall Superparamagnetic Iron Oxide Abstract Objective :In order to researching the preparation method of oct-USPIO. In order to researching the distribution of oct-USPIO in body. In order to evaluating the possibility of oct-USPIO as SSTR target tumor imaging agents. Methods:USPIO was prepared by precipitation, coupled with oct, and then labeled with 99 mTc,we got 99mTc -oct-USPIO. The size of 99mTc-oct-USPIO was measured with scanning electron microscopy, the radiation chemical purity of 99mTc-oct-USPIO was detected by centrifugation. The oct-USPIO were injected into mice via tail vein,than the liver, spleen, kidney and other organs or organizations were removed in different time, than its iron content was measured,and we computed %ID/g.The 99mTc-oct-USPIO were injected into breast cancer tumor-burdened mice via tail vein,in different time points SPECT imaging .Than we evaluated the possibility of it as breast cancer and other cancers SPECT imaging agents . Results:The size of USP