Trps1负向调控EMT对移植肝胆管纤维化的影响-普通外科学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 万方数据 万方数据 缩略语表 英文缩写 NABS 英文全称 nonanastomotic biliary strictures 中文全称 非吻合口胆管狭窄 BEC HIBEC CPRI EMT MET Trps1 biliary epithelial cell Human intrahepatic biliary epithelial cell cold preservation-reperfusion injury epithelial-mesenchymal transition mesenchymal -epithelial transition trichorhinophalangeal syndrome type 1 胆管上皮细胞 人肝内胆管上皮细胞 冷保存/再灌注损伤 上皮-间质转化 间质-上皮转化 发鼻指（趾）综合征 1 型 相关基因 E-cadherin CK19 Vimentin α-SMA DEPC FBS HBSS PMSF DAB EB EDTA PBS DTT SDS SDS E-cadherin Cytokeratin-19 Vimentin alpha smooth muscle actin Diethyl pyrocarbonate Fetal bovine serum Hank’s Balanced Salt Solution Phenylmethyl sulfonylfluoride Diaminobenzidine Ethidium bromide Ethylenediaminetetra-aceticacid Phosphate buffered saline Dithiothreitol Sodium dodecyl sulfate SDS-polyacrylamide gel 上皮钙粘附素 细胞角蛋白-19 波动蛋白 α-肌动蛋白 焦磷酸二乙酯 胎牛血清 Hank’s 平衡盐溶液 苯甲酸磺酰氟化物 二氨基联苯胺 溴乙锭 乙二胺四乙酸 磷酸盐缓冲盐水 二硫苏糖醇 十二烷基磺酸钠 SDS 聚丙烯酰胺凝胶 1 TBS Tris buffer solution Tris 盐缓冲液 TEMED tetramethylaethylenumdiamine 四甲基乙烯二胺 Tris Tris(hydro xymethy)aminomethan 三（羟甲基）氨基甲烷 AP Ammonium persulfate 过硫酸铵 EGTA Ethylenebistetra-acetic acid 乙二醇双四乙酸 UW University of Wisconsin solution 威斯康辛大学溶液 2 A preliminary study of Trps1 regulating biliary epithelial-mesenchymal transition and its roles in biliary fibrosis of the liver graft Abstract Backgrounds: Nonanastomotic biliary stricture (NABS) currently is one of the major biliary complications after liver transplantation, and might cause biliary cirrhosis, graft failure, re-transplantation, or death. It is the bottleneck that limits the improvement of liver transplantation efficacy. Pathologically, it is caused by the biliary fibrosis of the graft that results from ineffective or excessive repair of the injured bile duct epithelium. Studies show that the independent factor that affects the biliary fibrosis after liver transplantation is the cold preservation/reperfusion injury (CPRI); however, the mechanism needs to be further clarified. Previous studi