β连环素在脂溢性角化、鲍温病和鳞状细胞癌中的表达和意义-皮肤病与性病学专业论文.docxVIP

— — PAGE 1— 摘 要 β-连环素在脂溢性角化、鲍温病和鳞状细胞癌中 的表达和意义 研究生：屈园园 导师：普雄明教授 摘 要 目的：探讨β-连环素在脂溢性角化、鲍温病和鳞状细胞癌中的表达模式和意义。 方法：收集60例石蜡包埋福尔马林固定的组织标本，用β-连环素为抗体进行免疫组 化SP染色，组织学检查发现20例标本确诊为脂溢性角化，20例为鲍温病，20例为鳞 状细胞癌。检测β-连环素的膜染色水平和异常表达情况（包括膜表达缺失和异位表 达）。结果：β-连环素异常表达见于14例（23.3%）标本，在鳞状细胞癌中较脂溢性 角化（P﹤0.05）更常见，而脂溢性角化和鲍温病、鳞状细胞癌和鲍温病组间相比无 统计学意义；β-连环素在三组疾病的异常表达，大多数为膜表达缺失，也出现了膜 表达缺失和异位表达同时存在的现象；β-连环素的正常表达也存在两种形式，对β- 连环素在脂溢性角化、鲍温病的正常表达形式进行两样本率的比较（ P＞0.05），差异 无统计学意义。结论：β-连环素在表皮肿瘤的生长发育和侵袭转移中起着重要作用， 其在细胞膜的稳定表达对抑制肿瘤细胞非常重要，当β-连环素在细胞膜的表达弥漫 性缺失或异位表达可促进肿瘤细胞的转移和增殖。β-连环素可作为临床上一种免疫 组化指标判断表皮肿瘤的良恶性程度和预后；也为预防和治疗表皮肿瘤提供了分子 生物学线索。 关键词：β-连环素；脂溢性角化；鲍温病；鳞状细胞癌；免疫组化 新疆医科大学医学硕士学位论文 The Expression and Significance of Beta-catenin in Seborrheic Keratosis,Bowen’s Disease and Squamous Cell Carcinoma Postgraduate:Qu Yuan Yuan Supervisor:Prof.Pu Xiong Ming Abstract Objective: To investigate the expression pattern and significance of beta-catenin in seborrheric keratosis (SK), Bowen’s disease (BD), squamous cell carcinoma (SCC). Methods: Immunohistochemical staining was performed on 60 formalin- fixed paraffin-embedded tissue samples using antibodies to beta-catenin. On histoligical examination, 20 lesions were diagnosed as SK, 20 as BD and 20 as SCC. For beta-catenin, level of membranous staining and presence of aberrant (nuclear/cytoplasmic) localization were examined. Results: The abnormal expression of beta-catenin was observed in 14 specimens（23.3%）, more often in SCC than in SK (P 0.05), but no difference between BD and SCC , SK and BD was found. The abnormal expression of beta-catenin in 3 groups almostly were absent membranous beta-catenin expression, meanwhile absent membranous beta-catenin expression and aberrant beta-catenin cellular localization were also found together. There are 2 patterns in normal expression of beta-catenin, but no statistical difference between BD and SK was found in normal expression of beta-catenin （P＞0.05）. Conclusion: Aberrant expression of beta-catenin is closely related to the growth and inva