β-连环蛋白在膝关节原发性骨关节炎关节软骨中的表达及其意义-外科学专业论文.docxVIP

宁夏医科大 宁夏医科大学硕士研究生学位论文 中文摘要 II II 可能是 Wnt/β-catenin 信号通路活化后促进了炎性基因的转录，基质蛋白酶合成增加并被 激活，最终导致关节软骨的破坏。 关键词 膝关节，原发性骨关节炎，β-catenin，Mankin 分级，发病机制 宁夏医科大 宁夏医科大学硕士研究生学位论文 英文摘要 III III The expression and significance of β-catenin in the articular cartilage of knee primary Osteoarthritis ABSTRACT Objective To investigate the level of β-catenin in pathogenesis and progression of knee primary osteoarthritis(OA) by detecting the expression of β-catenin in different impaired articular cartilage of knee OA. Methods To collect 40 lesions cartilage specimens from adult knee primary OA patients who undergoing total knee arthroplasty and to collect 10 specimens from adult patients who suffering from amputation and femoral condylar fracture between October 2010 and May 2011. All cartilage-bone samples were taken out from femoral condylar. The decalcified paraffin-embedded sections were prepared and stained with Safranin O/fast green, then according to the modified version of the Mankin scale to divide the tissues: normal group （n=10),mild OA(n=12),severe OA(n=28)，the expression of β-catenin were detected in these samples by the Immunohistochemistry and Western-blot. Results 1. Histology Observation : Safranin O/fast green staining and Mankin scale: Normal group（n=10),mankin scores(0.50±0.48),all specimens come from adult patients who suffering from amputation and femoral condylar fracture; Mild OA(n=12),mankin scores(3.20±1.05), the cartilage characterisd by fissures within the superficial zone, chondrocyte count decreased and disordered, dupl icated tidemark; Severe OA(n=28), mankin scores(11.20±1.21),the cartilage characterisd by fissures extending into the deep zone, chondrocyte count obviously decreased and cluster, even the full-thickness cartilage defects. 2. β-catenin expression were detected by the Immunohistochemistry showed the normal cartilage was no immunoreactivity, mild OA expression level was 156.46 ± 8.16, severe OA expression level was 112.38±12.45,