川芎嗪对人肝星状细胞TGFβ1信号传导影响.docVIP

川芎嗪对人肝星状细胞TGFβ1信号传导影响 　　[摘要] 目的：探讨中药单体川芎嗪（TMP）对肝星状细胞（HSC）转化生长因子-β1（TGF-β1）信号传导的影响。通过检测TGF-β1及其Ⅰ型和Ⅱ型受体、Smad 3及Smad 7 mRNA表达的变化，探讨川芎嗪抗肝纤维化作用的相关机制。方法：体外培养人肝星状细胞， RT-PCR法检测HSC中TGF-β1及其Ⅰ型和Ⅱ型受体、Smad 3及Smad 7 mRNA表达。结果：与阴性对照组相比，TMP A、B、C各组均可显著降低TGF-β1 mRNA（P＜0.01）；TMP B、C组TβRⅠmRNA相对表达量降低，差异有统计学意义（P＜0.05或P＜0.01）；TMP B、C浓度组的TβRⅡmRNA、Smad 3 mRNA表达量显著降低（P＜0.01）；TMP B、C组可显著升高Smad 7 mRNA表达量（P＜0.01）。结论：川芎嗪可降低HSC中TGF-β1及其Ⅰ、Ⅱ型受体及Smad 3 mRNA表达，同时促进Smad7 mRNA表达，表明川芎嗪可抑制肝星状细胞的活化、增殖，这可能与阻断TGF-β/Smad通路的信号传导有关。 　　[关键词] 川芎嗪；肝纤维化；转化生长因子-β1；TβRⅠ；TβRⅡ；Smad 3；Smad 7 　　[中图分类号] R969 [文献标识码]A[文章编号]1674-4721（2011）08（b）-０19－０3 　　 　　Effects of tetramethylpyrazine on Transforming Growth Factor-β1 transduction of Human Hepatic Stellate Cells 　　FAN Yuhui1, ZHU Lin2, HUA Haiying3 　　1.Technician Training College of Medicine of Henan Province,Kaifeng 475003, China; 2.Department of Pharmacology, First Affiliated Hospital of Zhengzhou University, Henan Province, Kaifeng 475000, China; 3.Zhengzhou University Academy of Medical and Pharmaceutical Sciences,Henan Province, Kaifeng 475003, China 　　[Abstract] Objective: To investigate the effects of tetramethylpyrazine on Signal Transduction Induced by Transforming growth factor-β1 in Human Hepatic Stellate Cells. To explore the anti-hepatic fibrosis mechanisms of tetramethylpyrazine by studying the expressions of TGF-β1 mRNA, typeⅠ and typeⅡ TGF-β receptor mRNA, Smad 3 and Smad 7 mRNA. Methods: HSCs cultured in vitro were divided into negative control group, positive control group(cultured with Matrine 500 mg/L), experimental group(cultured with different concentrations of TMP (50 mg/L、100 mg/L、200 mg/L、400 mg/L、800 mg/L). The effect of TMP was evaluated of the level of TGF-β1, TβRⅠ, TβRⅡ, Smad 3 and Smad 7 mRNA by RT-PCR. Results: Compared with the negative control group, all of TMP groups of TGF-β1 mRNA expression of significant difference (P0.01); TMP B and C groups of TβRⅠ mRNA expression was decreased, the difference was significant (P0.05, P0.01)