失神经支配面肌形态学及超微结构变化的研究-整形与美容外科学专业论文.docxVIP

山西 山西医科大学硕士学位论文 PAGE PAGE 10 结论:1. 大鼠失面神经支配后，正常结构的眼轮匣肌超微结构随时间的延长呈 肿胀、排列紊乱、变性、增生等改变并呈进一步加重趋势且功能减退丧失。 2.大鼠失面神经支配后，随着失神经时间的延长，胶原纤维增多、肌肉 萎缩越明显，并且随着失面神经支配时间的延长呈递增趋势。 关键词:失神经支配:骨髓肌:超微结构:胶原纤维:大鼠 Study on the ultrastructure and morphology changes of facial muscle cells lack of nerve innnervation Abstract Objective:We aimed at the rats orbicularis oculi muscle cell ultrastructure and collagenous fiber changes of facial-nerve denervation observed by transmission electron microscope observation and Masson dyeing analysis at different periods in order to provide a theoretical basis for the best timing of facial-nerve surgical repair and making the treatment plan. Methods:70 normal adult Wistar rats with normal blink reflex,any male and female,weight 260 ± 20 g,were randomly divided into 7 groups,10 in each group. Prepared the facial nerve injury model.According to the different postoperative time,the rats were randomly divided into normal group, postoperative 1, 3days, 1, 2, 4, 6 weeks group. Based site was the orbicularis oculi muscle.Using transmission electron microscopy and computer image analyzer to observe the facial nerve collagen fiber and muscle and orbicularis oculi muscle ultrastructure cell area ratio changes. Results: 1. In normal muscle，myofibril arranged neatly , the z lines was clear, mitochondria distributed between myofibril uniformly and arranged ruly, unseen lysosome.In the postoperative 1week group, mitochondria, nucleus and sarcoplasmic reticulum changed not obviously. Within the 2 weeks group, mitochondria and sarcoplasmic reticulum had a mild expansion.The z line was clear, mitochondrial cristae became shorter.Nucleus also had a mild expansion.glycogen particles decreased. chromatin structure was loose. Lysosome was seen rarely.In the postoperative 4 weeks Group, muscle cells mitochondria was markedly swollen . The z line was fuzzy. Mitochondrial cristae became shorter or disappeared. Sarcoplasmic reticulum expanded. Nucleus changed not obviously