异氟醚对老年大鼠空间学习记忆能力及海马RhoA蛋白表达影响.docVIP

异氟醚对老年大鼠空间学习记忆能力及海马RhoA蛋白表达影响 　　[摘要] 目的 探讨异氟醚吸入麻醉对老年大鼠海马CA3区RhoA蛋白表达及空间学习记忆能力的影响。 方法 筛选出30只健康22月龄SD大鼠，随机分为对照组C组（n = 10）、麻醉组Ⅰ组（n = 10）和Ⅱ组（n = 10）。麻醉组以3%的异氟醚诱导，采用经口明视气管插管建立老年大鼠吸入麻醉模型，并以2%异氟醚维持麻醉2 h。C组大鼠不给任何麻醉药，只置于麻醉箱中吸入氧气2 h，氧流量为2 L/min。Ⅰ、Ⅱ组分别于麻醉后24、72 h进行旷场实验和Y-迷宫实验并计算成绩。比较三组大鼠学习记忆能力，并采用免疫组织化学法检测三组海马区RhoA蛋白表达。 结果 ①Y-迷宫实验：Ⅰ组的Y-迷宫学习次数[（70.50±16.25）次]明显多于Ⅱ组[（61.80±23.49）次]和C组[（49.20±8.56）次]（P 0.05）。旷场实验：三组的中央格停留时间、直立次数、跨格次数、总活动量次数比较，差异无统计学意义（P 0.05）。②免疫组织化学法：Ⅰ组海马CA3区RhoA阳性细胞计数（112.66±11.56）多于Ⅱ组（97.77±7.47）与C组（99.33±12.10）（P 0.05）。 结论 22月龄SD大鼠接受异氟醚麻醉后24 h空间学习记忆能力下降，于麻醉后72 h基本恢复，异氟醚麻醉后大鼠海马区Rho蛋白家族信号传导分子变化介导的神经突触形态学改变可能是短期空间学习记忆能力损伤的细胞学基础。 　　[关键词] 异氟醚；Y-迷宫；老年大鼠；RhoGTPases；RhoA 　　[中图分类号] R332 [文献标识码] A [文章编号] 1673-7210（2015）06（a）-0038-04 　　Affect of Isoflurane on spatial memory function and hippocampal RhoA expression in aged rats 　　ZHANG Wenchao1 WANG Geng1 HU Zhonghua2 DUAN Kaiming2 OUYANG Wen2▲ 　　1.Department of Anesthesiology， Beijing Jishuitan Hospital， Beijing 100035， China； 2.Department of Anesthesiology， the Third Xiangya Hospital of Central South University， Hunan Province， Changsha 410083， China 　　[Abstract] Objective To study affect of Isoflurane with inhalation anesthesia on hippocampal RhoA expression and spatial memory function in aged rats. Methods Thirty 22-month-old Sprague-Dawley rats were randomly assigned to control group （group C， 10 cases）， anesthesia group Ⅰ （10 cases） and anesthesia group Ⅱ （10 cases）. The inhalation anesthesia model of aged rat was established by using endotracheal intubation， 2% Isoflurane was applied to maintain anesthesia of 2 hours after anesthesia induction with 3% Isoflurane by inhalation in anesthesia groups. Any drug was not used in rat of group C， which inhaled 100% oxygen for 2 hours， oxygen flow rate was 2 L/min. Open-field experiment and Y-maze experiment were implemented， performance was calculated after anesthesia of 24， 72 hours in group Ⅰ and group Ⅱ. Learning and memory ability in rats of three groups wer