异荭草素对鼠伤寒沙门氏菌抑制作用及机理研究.docVIP

异荭草素对鼠伤寒沙门氏菌抑制作用及机理研究 　　摘要：研究了异荭草素对鼠伤寒沙门氏菌的抑菌活性，并初步探究了其抑菌机理，为开发高效低毒的抗菌药物提供理论依据。首先，采用滤纸片法测定异荭草素抑菌圈大小和抑菌率来确定异荭草素的抑菌活性；其次，再通过绘制生长曲线、测定细胞膜通透性和细胞内容物?B漏、总核酸来分析异荭草素的抑菌机理。结果表明，异荭草素浓度与对鼠伤寒沙门氏菌的抑菌率呈正相关；其抑菌机理可能是由于异荭草素抑制了细菌对数生长期的分裂速度，并导致细胞膜渗透性增加和细胞内的电解质外漏，影响细菌生长代谢，从而致死菌体细胞。上述结果说明，异荭草素作为天然抗菌剂有较好的开发应用潜力。 　　关键词：异荭草素；抑菌活性；机理 　　中图分类号：R284.2 文献标志码：A doi：10.16693/j.cnki.1671-9646（X）.2018.03.016 　　文章编号：1671-9646（2018）03a-0050-05 　　Abstract：This research showed the antibacterial activity of isoorientin on Salmonella typhimurium and its antibacterial mechanism，which provided a theoretical basis for the development of high efficiency and low toxicity antibacterial drugs. The size of inhibition zone and the rate of inhibition were determined by filter paper method to determine the antibacterial activity of isoorientin. Antibacterial mechanism of isoorientin was analysed by drawing growth curve and determining the cell membrane permeability and leakage of intracellular solution（water soluble protein and total nucleic acid）. The results showed that isoorientin concentration rate was positively correlated with inhibition of S. typhimurium. Antibacterial mechanism of isoorientin might be the inhibition of bacteria in logarithmic growth phase splitting speed which led to the increase of cell membrane permeability and the leakage of electrolyte inside the cell. The growth and metabolism of bacteria were affected and finally caused cell death. The results indicated that isoorientin has the potential of exploitation and application as natural antibacterial agent. 　　Key words：isoorientin；antibacterial activity；mechanism 　　0 引言 　　异荭草素（Isoorientin），又名3，4，5，7 -四羟基- 6 -吡喃葡萄糖基黄酮，是一种天然的黄酮类物质，其结构式如图1，因最早从荭草中分离发现而得名[1]。异荭草素主要存在于玉米、黑荞麦、竹笋、苦菜、山楂、百香果、西番莲等食物中[2-3]。研究发现，异荭草素具有清除自由基[4]、抑制癌细胞增殖[5-6]、抗氧化[7-8]、抗炎[9-10]、保肝[11-13]、调节免疫能力[14]等生物活性。天然抗菌成分一般为多酚或黄酮类物质，提取物己经广泛用于抑菌剂[15]，异荭草素作为一种水溶性黄酮，其对微生物的作用至今鲜有报道。试验以鼠伤寒沙门氏菌为供试菌，通过研究异荭草素对鼠伤寒沙门氏菌的抑菌效果、抑菌圈大小、最小抑菌浓度、生长曲线、蛋白质含量、细胞膜通透性及核酸含量等方面的变化来阐述异荭草素的抑菌效果及作用机制，为开发高