磺脲类药物对兔心肌缺血再灌注损伤后适应影响的研究.docVIP

磺脲类药物对兔心肌缺血再灌注损伤后适应影响的研究 　　[摘 要] 目的 观察磺脲类药物对兔心肌缺血再灌注损伤后适应影响及其机制。方法 将30只新西兰大白兔随机分为5组：（1）假手术组，仅穿线不作冠状动脉结扎；（2）缺血-再灌注组，缺血45分钟，再灌注2小时；（3）后适应组，缺血45分钟，然后行短暂灌注30秒，缺血30秒，共3次，再行全面的2小时再灌注；（4）格列美脲组，实验开始时外周静脉注射格列美脲02mg/kg，其余操作同后适应组；（5）格列本脲组，实验开始时外周注射格列本脲05mg/kg，其余操作同后适应组。实验结束时测各组血清肌钙蛋白含量及心肌梗死面积。结果 肌钙蛋白含量假手术组明显低于各手术组，后适应组与格列美脲组显著低于缺血再灌注组与格列本脲组，差异均有统计学意义（P005）。心肌梗死面积后适应组、格列美脲组明显低于缺血再灌注组及格列本脲组，差异有统计学意义（P005）。结论 格列本脲可消除后适应兔心肌缺血再灌注损伤的保护作用，而格列美脲却对后适应保护作用无影响。 　　[关键词] 心肌缺血再灌注损伤；后适应；磺脲类 　　中图分类号：R5422 文献标识码：A 文章编号：1009_816X（2014）02_0105_03 　　doi：103969/jissn1009_816ffects of Sulfonylurea on Myocardial Ischemia_reperfusion Injury in Rabbits. SHENG Xi_ling ，ZHU Yu_juan， XU Wen_bo， et al Department of cardiology， The First Jiaxing Hospital， Zhejiang 314000， China 　　[Abstract] Objective To explore the effects of sulfonylurea on myocardial ischemia_reperfusion injury in rabbits Methods 30 healthy New Zealand rabbits were randomly divided into 5 groups： （1）sham operation group（coronary artery ligation，CAL）； （2） ischemia reperfusion injury group （IRI）， ischemia for 45 minutes and reperfusion for 2 hours； （3） postconditioning （Post C） group， after ischemia for 45 minutes， 30 seconds reperfusion， 30 seconds occlusion of coronary artery and repeated for 3 times， then reperfusion for 2 hours； （4） glimepiride group， IV 02mg/kg of glimepiride through periphery vein， then the same process conducted as Post C group； （5）glibenclamide group， IV 05mg/kg of glibenclamide through periphery vein，then the same process conducted as Post C group The infarct size and the content of cTnI were measured at the end of experiment Results The content of cTnI in sham operation group was significantly lower compared with the other groups The content of cTnI in Post C and Glimepiride group were significantly lower than IRI and glibenclamide groups（P001） There was no significant difference between Post C and glimepiride group， IRI and glibenclamide group The infarct size of Post C and glimepiride group were