籽用南瓜疫病苗期抗性鉴定方法及抗病材料筛选的分析-identification of resistance to seed pumpkin blight at seedling stage and analysis of screening of resistance materials.docxVIP

硕士学位论文 籽用商瓜疫病苗期抗性鉴定方法及抗病材料筛选的研究 东北农业大学 摘要 南瓜疫病是由 Phytophthora capisici 引起的~种土传病害，是籽用南瓜生产上威胁 最大的病害之一。本论文主要研究疫霉菌以游动于包子在南瓜茵期接种的方法(接种浓度、 接种方法、苗龄、保湿时间)，确立南瓜疫病茵期抗性鉴定方法。 1.接种浓度共设 8 个水平，通过接种浓度与发病情况的关系图表，明确比较适宜 、·接种浓度范围是1250-7500 池子数1m)， 5000 抱子数Im1 为最适宜浓度。 2. 对 4 个不同茵龄进行接种比较，发现 2 叶期是接种的最佳茵龄。 3. 设 3 种接种方法，其中灌根法最实用。而注射法发病快而且程度重，根部切伤 灌根法对植株造成伤害，影响正常生长。 4. 保湿时间对发病的影响很大，时间短，不能充分表现症状:时间过长，影响幼 苗的正常生长:接种后保湿 48 小时最适宜 。72 小时后，发病充分，症状稳定，可以开 始调查病情。 5. 采用正交设计 L9 (3勺，分析发现保湿时间是影响接种效果的主要因子，决定发 病程度。 6. 利用以上研究结果对 29 份南瓜试材进行抗病性鉴定，筛选出抗南瓜疫病材料 6 份。 7. 对抗性不同的南瓜材料进行酶活性分析，发现健康植株中， POD ，PPO 活性差 异都不明显。其抗病性与 POD 和 PPO 活性呈正相关.病菌入侵后 POD 活性在感病材料 中增长幅度大于在抗筑材料中的增长幅度; PPO 活性在抗病材料中的增长幅度大子在感 病材料中的增长幅度八 关键词:南瓜疫病接种鉴定抗源筛选 POD PPO 东北农业大学轩用南瓜疫病菌期抗性鉴定方法及抗病材料筛选的研究硕士学位论文 东北农业大学 轩用南瓜疫病菌期抗性鉴定方法及抗病材料筛选的研究 硕士学位论文 Identification and screening 00 the resistance to squash blight in seeding stage Abstract Squash bIight ís a soil-spreading disease caused by Phytophthora capisici. Jt ìs one of diseases which put the most threat on the squash production. The influence of P. capisici affecting the seedling squash inoculated by the inoculating method of zoospore seedling squash inoculating ，such as inoculation density，ìnoculating way，seedling stages and preserving moisture time was studied ìn thìs paper. The results showed: 1. Inoculating density is designed 8 levels by setting up the figure and the table between the inoculating density and disease it is found that the range of comparatively suitable inoculating density is 1250-7500 spore Iml and the most appropriate density is 5000 spore/m 1. [t is found that the best seedling stage ?s 2 leaves comparing 4 seedling stage. There are 3 inoculating ways. Away them ，pouring the root is the most suitable; injecting causes the disease quickly and seriously; cutting root gives the plants injury and affects the growth. Preserving moisture time has great inf1uence on infection. But if the tíme is too short，symptom cannot been showed complete