转基因内皮细胞修饰血管内支架的实验分析-experimental analysis of transgenic endothelial cells modified endovascular stents.docx

PAGE PAGE IV III III 和扫描电镜下观察，细胞在支架表面粘附生长，荧光显微镜检测细胞高表达 VEGF 蛋白；5) 体外流动腔实验检测发现，转基因内皮细胞在模拟体内传输过程中，将 有部分细胞损失，但是在不同的切应力作用下，转基因内皮细胞又很快的恢复生 长；6) 动物实验结果发现与两个对照组相比，细胞包被支架均极显著的抑制了内 膜增生和降低了支架内再狭窄。 综上所述，本研究初步解决了血管内支架的细胞种植方法，提出一种组织工 程血管内支架的实验室制备方法和检测方法，得到的相关实验结果为细胞种植防 治血管内支架再狭窄的临床应用提供实验依据。内皮细胞种植支架预防再狭窄的 研究已显示出诱人的前景，随着进一步的深入研究，相信将会开辟出一片新的领 域。 关键词：支架内再狭窄，内皮细胞，基因转染，血管内皮生长因子，旋转培养 ABSTRACT Vascular stent implant in coronary artery is critical treatment to the coronary heart disease. However, neointima hyperplasia still couldn’t be inhibited through stent embedding. The incidence rate of In-stent restenosis (ISR) is still to remain 13-20%. Most studies lay particular emphasis on drug-eluting stent (EDS), although the uncovered struts may be prone to late thrombosis after the discontinuation of antiplatelet therapy, and also the other potential complications such as delayed endothelialization, aneurysm because of the coating materials. So it may be an effective process to work out a solution by promoting re-endothelialization and injure healing of endothelial layer. Therefore, the integrity and functional activity of the endothelial monolayer play a crucial role in the prevention of thrombosis and in-stent restenosis. More and more researches illustrated that the coating endothelial cells (ECs) of stents would repair the endothelial layer of the injured arterial wall and suppress intima hyperplasy. ECs cultured in vitro may lost some biological characteristics and lead to slow growth due to the changes of environment. Combination of gene therapy is expected to enhance cell proliferation by stable transfection with vascular endothelial growth factor. Combination of dynamic culture is expected to promote the adherence and growth of endothelial cells on the vascular stent surface. Combination of the simulation experiments of circulatory system is expected to control the cells’ loss in the process of stent implantation and improve the reliability of animal experiments results. This stu