景天三七茎叶化学成分研究.docVIP

景天三七茎叶化学成分研究 　　[摘要] 目的 对景天三七（Sedum aizoon L.）茎叶的化学成分进行研究。 方法 采用70%乙醇对景天三七干燥茎叶进行提取，乙醇总浸膏用水混悬，依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取，利用硅?z柱色谱，Sephadex LH-20柱色谱和制备高效液相色谱等手段进行分离纯化，根据理化性质和波谱数据对化合物进行结构鉴定。 结果 从景天三七茎叶的70%乙醇提取物的正丁醇层萃取物中分离得到9个化合物，分别鉴定为山柰酚-3-O-β-D-吡喃葡萄糖苷（1）、杨梅素-3-O-α-L-吡喃阿拉伯糖苷（2）、槲皮素-3-O-α-L-吡喃阿拉伯糖苷（3）、草质素-3-O-α-L-吡喃阿拉伯糖苷（4）、woodorien（5）、vanilloloside（6）、benzy-1-O-β-D-glucopyranoside（7）、苯乙醇-O-β-D-葡萄糖苷（8）、benzylethyl-O-β-D-xylopyranosyl-（1→2）-β-D-glucopyranoside（9）。 结论 化合物4为未见文献报道的新化合物，化合物6～9均为首次从景天属植物中分离得到。 　　[关键词] 景天三七；茎叶；化学成分；草质素-3-O-α-L-吡喃阿拉伯糖苷；杨梅素-3-O-α-L-吡喃阿拉伯糖苷 　　[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210（2017）09（c）-0033-04 　　[Abstract] Objective To study the chemical constituents in the stems and leaves of Sedum aizoon L. Methods The stems and leaves of Sedum aizoon L. was extracted with 70% ethyl alcohol to give ethanol extracts， which was further diluted by H2O and extracted with petroleum ether， chloroform， EtOAc and n-BuOH， respectively. They were isolated and purified by silica gel column chromatography， Sephadex LH-20 gel column chromatography and high performance liquid chromatography methods. The structures were elucidated based on the physic-chemical characters and spectral analysis. Results Nine compounds were obtained from n-BuOH extracts of 70% ethyl alcohol extracts from stems and leaves of Sedum aizoon L.， which was kaempferol-3-O-β-D-glucopyranoside （1）， myricetin-3-O-α-L-arabinopyranoside （2）， quercetin-3-O-α-L-arabinopyranoside （3）， herbacetin-3-O-α-L-arabinopyranoside （4）， woodorien （5）， vanilloloside （6）， benzy-1-O-β-D-glucopyranoside （7）， benzylethyl-O-β-D-glucopyranoside （8）， benzylethyl-O-β-D-xylopyranosyl-（1→2）-O-β-D-glucopyranoside （9）， respectively. Conclusion Compound 4 is a new compound， which has not yet been reported. Compounds 6-9 are isolated from genus Sedum for the first time. 　　[Key words] Sedum aizoon L.； Stems and leaves； Chemical constituents； Herbacetin-3-O-α-L-arabinopyranoside； Myricetin-3-O-α-L-arabinopyranoside 　　景天三七（Sedum