光源在园艺与药用植物和人工虫草室内培养中应用［.pptxVIP

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光源在园艺与药用植物和人工虫草室内培养中应用［.pptx

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光源在园艺与药用植物和人工虫草室内培养中应用［

植物的种子萌发、幼苗生长、形态建成、开花结果等过程都需要光不同的生物及不同生长发育期需要不同的光质与光强光在植物与食用菌等的室内培养中广泛应用光照降温研究内容非洲菊组织培养不定芽诱导、芽增殖和生根铁皮石斛和金钗石斛组培繁殖原球茎增殖、生长、分化虫草子实体形成,生长,功能成分子实体的诱导形成生长：高、鲜重、干重生物活性物质: 虫草素,多糖,类胡萝卜素Shoot multiplication procedureRedifferentiationDedifferentiationcallusExplant:Adventitious budsLED treatmentMultiplication DivisionPlantletsRootingCluster shootsSingle shootFresh weight and dry weightCompared to WFL, no significant differences were observed in the treatments of V1,V2, V5 and V6. V3 and V4 decreased the FW of the shoots. V6 increased dry weight significantly.Height and number of shootsAll LEDs could increase the height of shoots except V5. But No.ofshoots/explant had no significant different.Chlorophyll contentWAll LEDs induced chlorophyll biosynthesis significantly, compared with WFLV1V2V3V4V5V6WFL35 days cultured in vitro under LED V1V4 V3V6 V2 V5WFL35 days cultured in vitro under LEDResults — Root inductionRedifferentiationDedifferentiationcallusExplant:Adventitious budsReceptacle Multiplication DivisionPlantletsRootingCluster shoots Root induction under LED Single shootFresh weight and Dry weightV3 treatment decreased FW and DW.V2 treatment increased FW and DW significantly.Shoot height and root numberHeights of shoots under V1, V2, V3 and V4 were significantly higher thancontrol.V2 had the most number of roots compared with others.Root activity and maximum root lengthV3, V4 and V5 promoted the root activity significantly.V2 increased the maximum root length.V1V2V3V4V5V6WFLSeedlings 35 d after rooting inductionResults — TransplantationHeight (cm)No. of leavesSurvival rate9.46±0.22 a9.77±0.22 a8.19±0.34 b9.54±0.30 a8.02±0.27 b8.25±0.48 b7.09±0.23 c5.19±0.23 b4.94±0.11 b6.06±0.19 a6.00±0.35 a5.31±0.24 b6.19±0.20 a4.60±0.27 bV1V2V3V4V5V6WFL100%100%100%100%100%100%100%15 days after transplantation, no difference on seedlings survival rates.All LED treated seedlings were significant higher and number of leaves increased.V4 V1V5 V2V6 V3WFLTransplanted seedlings grown for 15 daysCo