生醋莪术对大鼠免疫性肝纤维化及HSC―T6增殖和α―SMA,ProcollagenⅠ表达影响.docVIP

生醋莪术对大鼠免疫性肝纤维化及HSC―T6增殖和α―SMA,ProcollagenⅠ表达影响 　　[摘要] 比?^生、醋莪术对猪血清所致大鼠免疫性肝纤维化及对活化的肝星状细胞（HSC-T6）增殖和α-SMA，ProcollagenⅠ表达的影响。采用腹腔注射猪血清0.5 mL/只，每周2次，共14周制备大鼠免疫性肝纤维化模型。观察生、醋莪术（0.95，1.90 g?kg-1）对大鼠血清ALT，AST，PC-Ⅲ，IV-C，LN，HA和肝组织HYP，MDA的表达水平；采用HE染色观察大鼠肝组织病理改变情况，Masson染色和天狼猩红染色观察大鼠肝组织中胶原表达情况；体外培养HSC-T6细胞，MTT法测定不同浓度生、醋莪术含药血清作用12，24，36，48 h对HSC-T6增殖的影响；采用Real-time PCR法检测各组α-SMA和ProcollagenⅠ表达情况。结果显示，给药生、醋莪术后，大鼠血清ALT，AST，PC-Ⅲ，IV-C，LN，HA表达水平均下降，与生莪术组相比，醋莪术组作用效果优于生莪术组；生、醋莪术含药血清各剂量组均能抑制HSC-T6的增殖，呈现一定的量效和时效关系；各给药组作用24 h后，HSC-T6中α-SMA和Procollagen Ⅰ表达水平降低，且醋莪术组降低更显著。生、醋莪术均能不同程度减轻肝纤维化，其抗肝纤维化机制可能与抑制HSC-T6增殖，减少细胞外基质的生成并促进其降解有关。 　　[关键词] 莪术；醋莪术；肝纤维化；肝星状细胞；Real-time PCR；α-平滑肌肌动蛋白；Procollagen Ⅰ 　　[Abstract] To compare the effects of Curcumae Rhizoma/vinegar-processed Curcumae Rhizomaon immune hepatic fibrosis， proliferation of HSC-T6， and expressions of α-SMA and Procollagen I. The immunological liver fibrosis model was prepared through intraperitoneal injection with porcine serum 0.5 mL in each rat， twice a week， for 14 weeks. Expressions of serum ALT， AST， PC-Ⅲ， IV-C， LN， HA and HYP， MDA in liver tissues were observed after administration of Curcumae Rhizoma/vinegar-processed Curcumae Rhizoma （0.95， 1.90 g ? kg-1）. The pathological changes in liver tissues were observed by HE staining. Masson staining and Sirius red staining were used to observe the expression of collagen in rat liver. HSC-T6 was cultured， and the proliferation of HSC-T6 was determined by MTT assay at different concentrations in 12， 24， 36， 48 h. The expressions of α-SMA and Procollagen I were detected by Real-time PCR. The results showed that expressions of serum ALT， AST， PC-Ⅲ， IV-C， LN and HA in Curcumae Rhizoma/vinegar-processed Curcumae Rhizoma groups （0.95， 1.90 g?kg-1） were significantly lower than model group；in terms of effect， vinegar-processed Curcumae Rhizoma group was superior to Curcumae Rhizoma group. Curcumae Rhizoma/vinegar-processed Curcumae Rhizoma containing serum could inhibit the proliferation of