熊果酸诱导慢性粒细胞白血病细胞凋亡过程中活性氧生物学功能探讨.docVIP

熊果酸诱导慢性粒细胞白血病细胞凋亡过程中活性氧生物学功能探讨 　　[摘要] 目的 研究熊果酸对慢性粒细胞白血病细胞株K562的凋亡及其过程中活性氧自由基的作用。 方法 不同浓度UA处理后用显微镜观察K562细胞的形态学变化；用Annexin V/PI双染色法检测凋亡；用DCFH-DA法检测活性氧自由基的水平。 结果 20μmol/L浓度组可见细胞变形、坏死，40μmol/L浓度组可见细胞变形、核固缩、核碎裂和胞膜突起等现象。10、20、40μmol/L作用48h后K562细胞凋亡呈剂量依赖关系，凋亡率分别为21.66%、40.55%、70.21%，随着UA浓度的增高细胞凋亡率也增高，差异具有统计学意义（P0.01）。熊果酸能显著增高K562细胞内的活性氧水平，且活性氧水平随着熊果酸的浓度增加而升高。平均荧光强度由48.96升至78.23，差异具有统计学意义（P0.01）。 结论 熊果酸可诱导慢性粒细胞白血病凋亡，且活性氧自由基的水平在凋亡诱导过程中起了重要作用。 　　[关键词] 熊果酸；慢性粒细胞白血病；凋亡；活性氧自由基 　　[中图分类号] R733.72 [文献标识码] A [文章编号] 2095-0616（2015）15-27-04 　　[Abstract] Objective To study the effect of UA on apoptosis of ROS cell line K562 and its process. Methods Different concentrations of UA treatment after microscope was used to observe the morphological changes of K562 cells； annexin V/PI double staining method for the detection of apoptosis； variations method was used to detect the reactive oxygen species （ROS） level. Results In the 20μmol/L concentration group， the deformation and necrosis of the cells were found， and the deformation of the cells was 40μmol/L. 10， 20， 40μmol/L for 48h after apoptosis of K562 cells was dose dependent， the apoptosis rate was 21.66%， 40.55%， 70.21%， with UA concentration increased cell apoptosis rate increased. The difference is statistically significant （P0.01）. UA can significantly increase the level of reactive oxygen species in K562 cells， and the level of reactive oxygen species increases with the increase of UA concentration. The average fluorescence intensity was 48.96 to 78.23， and the difference was statistically significant （P0.01）. Conclusion UA can induce the apoptosis of chronic myeloid leukemia， and the level of reactive oxygen species plays an important role in the process of apoptosis induction. 　　[Key words] UA； Chronic myeloid leukemia； Apoptosis； Reactive oxygen species 　　熊果酸（UA）属于五环三萜类化合物，广泛分布于自然界中[1-5]。UA对多种致癌和促癌物质均有不同程度的抵抗作用，并且能够抑制多种实体肿瘤细胞的生长[1-5]。Ning等[6]（2012年）探讨了来源于髓系的人白血病细胞U937中由UA诱导的细胞凋亡过程，结果表明，PKB失活和JNK活化在UA引起的细胞凋亡中发挥着重要的作用。活性氧自由