猪圆环病毒2型感染对猪瘟疫苗免疫效果影响.docVIP

猪圆环病毒2型感染对猪瘟疫苗免疫效果影响 　　摘要：为探索在猪圆环病毒2型（PCV-2）阳性猪场免疫猪圆环病毒疫苗对猪场实际临床生产中猪瘟疫苗免疫效果的影响，遂将某规模化猪场的一栋存在PCV-2感染的150头产房仔猪随机分为试验组和对照组，每组75头，试验组在14日龄免疫某进口PCV-2杆状病毒载体灭活疫苗，对照组不免疫，并分别在PCV-2疫苗免疫前和猪瘟疫苗二免后30天每组随机采血18头仔猪检测猪瘟抗体。结果显示：PCV-2疫苗免疫前两组仔猪的猪瘟抗体水平无明显差异，猪瘟疫苗二免后30天，试验组的猪瘟抗体平均值和猪瘟抗体离散度均显著好于对照组。表明，PCV-2的感染会对猪的免疫系统造成损害从而抑制猪瘟疫苗的免疫应答，在PCV-2阳性猪场免疫猪圆环病毒疫苗能够显著增强猪瘟疫苗的免疫效果。 　　关键词：猪圆环病毒2型；猪瘟抗体；跟踪监测；强化免疫；应用 　　中图分类号：S852.65+1 文献标识号：A 文章编号：1001-4942（2015）08-0086-03 　　Abstract In order to explore the influence of Porcine Circovirus Type 2 （PCV-2 ） vaccination on the immune effect of Classical Swine Fever （CSF） vaccine in the PCV-2 positive pig farms during practical clinical production， 150 piglets in a farrowing house of a large pig farm which had been confirmed to be PCV-2 positive were randomly divided into two groups， namely， test group and contrast group， and each 75 piglets. The piglets in the test group were immunized with the imported PCV-2 baculovirus vector inactivated vaccine when they were 14 days old. Then， 18 piglets were randomly selected from each group before PCV-2 vaccination and 30 days after CSF revaccination respectively. The detection results showed that： there was no difference between the two groups in CSF antibody level before PCV-2 vaccination； 30 days after CSF revaccination， the average value and dispersion of CSF antibody in the test group were significantly higher than those of the contrast group. It indicated that the immune system of pigs could be damaged by PCV-2 infection and the immune response of CSF vaccine was suppressed. In addition， the immune effect of CSF vaccine could be enhanced by immunizing with PCV-2 vaccine in the PCV-2 positive pig farms. 　　Key words Porcine Circovirus Types 2（PCV-2）；CSF antibody； Tracking monitoring； Supplementary immunization； Application ??　猪圆环病毒2型（Porcine circovirus type 2，PCV-2）引起的猪圆环病毒相关疾病（Porcine circovirus type 2 associated diseases，PCVAD）是危害当今全球养猪生产最主要的免疫抑制性疾病。目前PCV-2在各地猪场内感染十分普遍，其感染率可高达96%以上[1]。研究表明，PCV-2感染可导致猪的免疫器官损