溶血温度以及储存时间对肝功能生化检测影响.docVIP

溶血温度以及储存时间对肝功能生化检测影响 　　[摘要] 目的 评价标本溶血、温度以及储存时间对肝功能生化检测效果的影响。 方法 选取2011年3月~2013年7月来我中心进行健康体检的正常人群90例，随机平均分为A、B、C三组，研究溶血、温度以及储存时间对ALT（谷丙转氨酶）、AST（谷草转氨酶）、TBiL（总胆红素）和DBiL（直接胆红素）的影响。 结果 A组中比较发现溶血组与不溶血组AST、TBiL和DBiL差异有统计学意义（P＜0.05），ALT比较差异无统计学意义（P＞0.05）；B组与C组中比较差异均无统计学意义（P＞0.05）。 结论 在肝功能生化检测中，溶血能影响AST、TBiL和DBiL水平，温度以及储存时间对检验结果无影响，所以在检验过程中应注意防止样本溶血。 　　[关键词] 肝功能；溶血；生化检测效果 　　[中图分类号] R446.11#8195;#8195;#8195;[文献标识码] B#8195;#8195;#8195;[文章编号] 2095-0616（2014）11-126-03 　　 　　Impact of hemolysis, temperature and storage time on liver function biochemical test 　　ZHU#8195;Guiying#8195;#8195;GUO#8195;Kaihua 　　Occupational Health Service Center, Zhaoqing Municipal Center for Disease Control and Prevention, Zhaoqing 526020, China 　　[Abstract] Objective To evaluate the impact of hemolysis, temperature and storage time on liver function biochemical test. Methods 90 healthy populations receiving physical examination in our center from Mar 2011 to Jul 2013 were selected and randomly assigned to Group A, Group B and Group C to study the impact of hemolysis, temperature and storage time on ALT(glutamic-pyruvic transaminase), AST(glutamic-oxalacetic transaminase), TBiL (total bilirubin) and DBiL (direct bilirubin). Results In Group A, the hemolysis subgroup and non-hemolysis subgroup had difference in AST, TBiL and DBiL, with statistical significance (P＜0.05), but no statistical difference in ALT (P＞0.05). The difference had no statistical significance in Group B or Group C (P＞0.05). Conclusion Hemolysis can affect AST, TBiL and DBiL levels in liver function biochemical tests, but the temperature and storage time have no impact on test results. Therefore, it is proposed to prevent sample hemolysis during tests. 　　[Key words] Liver function; Hemolysis; Biochemical test results 　　我国作为酒文化大国，饮酒人数较多，近年来随着经济发展，因饮酒而导致肝硬化、肝癌的患者进一步增多，因此常规体检对于肝功能的检测准确率要求较高[1]。目前临床上主要应用血生化检测ALT、AST、TBiL和DBiL等指标判断肝功能，检测过程中影响因素较多[2]。我中心通过对来健康体检的正常人进行分组研究，评价标本溶血、温度以及储存时间对肝功能生化检测效果的影响，取得了较好的效果，现报道如下。 　　 　　1#8195