男性不育患者年龄与精子DNA碎片和精液常规参数相关性分析.docVIP

男性不育患者年龄与精子DNA碎片和精液常规参数相关性分析 　　【摘 要】 目的:分析男性不育患者年龄与精子DNA碎片率和精液常规参数的相关性。方法：按照年龄将85例男性不育患者分为三组。20~29岁组(n=31)，30~35岁组（n=28），36~50组（n=26），采用染色质扩散(SCD)实验检测精子DNA碎片率，按照世界卫生组织标准检测精液常规各参数。采用SPSS 13. 0 软件包进行统计处理,分析精子DNA碎片率和精子参数在各组的差异及年龄与精子DNA碎片率和精液参数的相关性。结果：年龄与精子活力存在负相关关系( r= -2.31, P0.05) ，但与精子密度，精子形态和精子DNA碎片无相关性。精子密度与精子形态存在正相关关系（r=0.528, P0.01），与精子活力存在正相关关系，（r=0.31, P0.01）。精子DNA碎片率与精液常规各参数无相关性。结论：随着男性年龄的增长，精子的活力可能降低,并影响男性的生育力，精子DFI与精液常规各参数无相关性。故精子DNA碎片检测可作为精液常规分析的补充,进一步反映男性的生育能力。 　　【关键词】 男性不育； 年龄； 精子DNA碎片； 精液常规参数； 分析 　　 　　Correlation of age, sperm DNA fragmentation with semen parameters in infertile males 　　YANG Yi?1, JIANG Hui?1， ZHANG Haijiao?2, HONG Kai?1 TANG Wenhao?1， ZHAO Lianming?1， MAO Jiaming?1， LIU Defeng?1 　　1 Department of Urology, the Third hospital of Peking University, Beijing 100191, China 　　2 Department of Hematology/oneology, Jilinunversity First Hospital, Changchun 130000, China 　　【Abstract】 Objectives: To analyze the correlation of age and sperm DNA fragmentation with semen parameters in infertile males. Methods: Eighty-five cases of infertile males were divided by age into three groups: 20~29 age group (n=31), 30~35 age group (n=28), 36~50 group, (n=26). Sperm DNA fragmentation was measured by sperm chromatin dispersion (SCD). Semen conventional analysis was performed based on WHO criteria (1999). Results: Age negatively correlated with sperm motility (r=-2.31,P0.05), but was independent of sperm fragmentation, sperm concentration and sperm morphology. Sperm concentration was found to have significant positive correlation with sperm morphology（r=0.528,P0.01）and sperm motility（r=0.31,P0.01）.No correlations were found. between sperm DNA fragmentation and semen parameters. Conclusion: Increased age of infertile men is associated with a decline in sperm motility and would exert a negative influence on male fertility. Measurement of sperm DNA fragmentation can be complementary to sperm parameters in the evaluation of male fertility. 　　【Key words】 M