癌相关成纤维细胞中Gal―1表达通过integrin β1促进胃癌细胞侵袭机制研究.docVIP

癌相关成纤维细胞中Gal―1表达通过integrin β1促进胃癌细胞侵袭机制研究 　　[摘要] 目的 研究癌相?P成纤维细胞中Gal-1表达通过integrin β1促进胃癌细胞侵袭的机制。 方法 高表达Gal-1的胃癌CAFs采用原代培养法，通过CAFs与胃癌细胞共培养、siRNA转染、抑制Gal-1/封闭integrin β1表达、细胞侵袭能力实验、侵袭能力影响实验和IHC实验等技术，研究胃癌细胞侵袭迁移能力受胃癌CAFs中Gal-1表达的影响程度及作用机制。分析高表达Gal-1 的CAFs对癌细胞迁移能力和侵袭能力的影响，比较Gal-1和integrin β1免疫组化实验结果。 结果 CAFs共培养使得胃癌细胞侵袭和迁移能力得到明显提升，Gal-1的表达遭到siRNA抑制后，CAFs对迁移能力和侵袭能力的促进作用得到有效抑制。Gal-1主要在胃癌细胞的间质成纤维细胞中表达，integrin β1主要在胃癌细胞的细胞膜中表达。Gal-1和integrin β1在胃癌中的阳性率分别为56.67%（51/90）、43.33%（39/90），并且其表达与淋巴结转移、远处转移、TNM分期差异具有统计学意义（P0.05）。 结论 CAFs表达Gal-1并通过促进胃癌细胞integrin β1表达的形式提高胃癌细胞的侵袭迁移能力，指明今后胃癌的治疗和相关预后的研究方向，表现出一定的理论研究价值。 　　[关键词] 癌相关成纤维细胞；Gal-1；integrin β1；胃癌；侵袭 　　[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-9701（2016）34-0001-03 　　Study on the mechanism of expression of Gal-1 in cancer-related fibroblasts promoting the invasion of gastric cancer cells via integrin β1 　　NI Haibin1 SUN Yuanshui1 WANG Fengyong1 XU Ji2 HE Xujun2 CHEN Jian1 WU Qi1 WU Jinfeng1 　　1.Department of Gastrointestinal Surgery， Tongde Hospital of Zhejiang Province， Hangzhou 310012， China； 2.Department of Gastrointestinal Surgery， Zhejiang Peoples Hospital， Hangzhou 310014， China 　　[Abstract] Objective To study the mechanism of expression of Gal-1 in cancer-related fibroblasts promoting the invasion of gastric cancer cells via integrin β1. Methods Gastric cancer CAFs with highly expressed Gal-1 were primarily cultured， co-cultured with gastric cancer cells， transfected with siRNA， inhibited Gal-1 expression/blocked integrin β1 expression， given cell invasiveness test， given invasiveness test and IHC experiment technology. The effect and mechanism of gastric cancer cell invasion and migration on the expression of Gal-1 in gastric cancer CAFs were studied. The effect of CAFs with highly expressed Gal-1 on the migration and invasiveness of cancer cells was analyzed， and the results of Gal-1 and integrin β1 immunohistochemistry were compared. Results The co-culture of CAFs was able to enhance the invasion and migration of gas