种子引发对NaCl胁迫下通关藤种子萌发及幼苗生理特性影响.docVIP

种子引发对NaCl胁迫下通关藤种子萌发及幼苗生理特性影响 　　[摘要]以采自云南省马关县的当年成熟通关藤种子为实验材料，分别研究了不同浓度的KNO3-KH2PO4 （K-K），GA3，PEG-6000，NaCl 4种试剂引发处理对通关藤种子在高盐胁迫（0.8%NaCl）逆境下发芽及幼苗素质的影响，分别测定了发芽势、发芽率、 发芽指数、根长、茎长以及侧根数。并测定了通关藤叶片中丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性的变化及可溶性蛋白含量的变化。结果表明，0.80%NaCl胁迫下，通关藤种子萌发受到显著抑制，经不同浓度的4种试剂引发处理后，均不同程度地提高了盐胁迫条件下通关藤种子的发芽势和发芽率等指标，均显著提高了SOD，CAT活性、增加了可溶性蛋白的含量、降低了叶片中MDA量。其中，经1.20% KNO3-KH2PO4 溶液引发处理的效果最好，引发处理后，通关藤种子发芽率从52.67%提高到87.33%，SOD活性从138.01提高到219.44;其次是300 mg?L-1GA3溶液引发处理的种子，其发芽率从52.67%提高到80.67%，SOD活性从138.01提高到444.61。 　　[关键词]通关藤;种子引发;盐胁迫;种子萌发;生理特性 　　Effects of seed priming on physiology of seed germination and seeding 　　growth of Marsdenia tenacissima under NaCl stress 　　XIAO Xue-fenglt;supgt;1lt;/supgt; ， LIU Lilt;supgt;1lt;/supgt;， GUO Qiao-sheng1*， LI Chaolt;supgt;1lt;/supgt;， WANG Ping-lilt;supgt;2lt;/supgt;， YANG Sheng-chaolt;supgt;3lt;/supgt;， HANG Yue-yu4* 　　（1.Institute of Chinese Medicinal Materials， Nanjing Agricultural University， Nanjing 210095， China; 　　2. Yunnan Xintong Phytopharma Ceutical Co.， Ltd.， Mengzi 661100， China; 　　3. Yunnan Agricultural University， Kunming 605201， China; 　　4. Institute of Botany Jiangsu Province and the Chinese Academy of Science， Nanjing 210014， China） 　　[Abstract]To offer the reference and method for salt damage in the cultivation of Marsdenia tenacissima， the seeds of M. tenacissima collected from Maguan city （Yunnan province） were taken as the test materials to study the effects of different priming materials on improving germination and growth under high-level salt stress condition. Four different treatments， which were GA3， KNO3-KH2PO4， PEG-6000， NaCl， combined with ANOVA were applied to test the performance of germination energy， germination percentage， germination index， MDA， SOD， and CAT. The results showed that the seed germination was obviously inhibited under salt stress and the soaked seeds with different priming materials could alleviate the damage of salt stress. Under these treatments， the activities of SOD，