砒霜对MRLlpr自发性狼疮小鼠外周血单个核细胞DNMT1及CD11a表达影响.docVIP

砒霜对MRLlpr自发性狼疮小鼠外周血单个核细胞DNMT1及CD11a表达影响 　　摘 要：目的：探讨砒霜(三氧化二砷 ATO)对MRL/lpr自发性狼疮小鼠外周血单个核细胞（PBMC）中DNA甲基转移酶1（DNMT1）和黏附分子淋巴细胞功能相关抗原-1（LFA-1）的亚基CD11a表达的影响及其可能作用机制。方法：20周龄（发病期）MRL/lpr狼疮小鼠24只，设C57BL正常小鼠24只做参照，随机平分两组，即治疗组(ATO组)和对照组(NS组)，治疗组ATO 0.4mg·kg-1·d-1隔日腹腔注射，对照组隔日腹腔注射同等量生理盐水，疗程为60天，采用RT-PCR检测两组小鼠PBMC的DNMT1及CD11a转录水平的表达情况。结果：MRL/lpr小鼠治疗组中DNMT1-mRNA的灰度比值对照组增高（P＜0.05），MRL/lpr小鼠治疗组中CD11a-mRNA的灰度比值较对照组降低（P＜0.05）。结论：砒霜能逆转系统性红斑狼疮(SLE)的低甲基化状态。 　　关键词：砒霜；系统性红斑狼疮；DNA甲基转移酶1；CD11a 　　 中图分类号：R593.24文献标识码：A文章编号：1673-7717(2011)12-2750-03 　　Effects of Arsenic Trioxide on the Expression of DNA Methyltransferase 1 and CD11a 　　Genes in Peripheral Blood Mononuclear Cells of Lupus Nephritis-prone MRL/lpr Mice 　　WANG Hong1，XIE Qipeng2 　　(1.Department of Nephrology,Rheumatology Immunology，Second Affiliated Hospital of Wenzhou Medical College，Wenzhou 325000， 　　Zhejiang,China;2.Climical Lab,Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,Zhejiang,China 　　 Abstract:Objective：To investigate the ATO regulatory mechanism on the expression of DNA Methyltransferase 1-mRNA and CD11a-mRNA in lupus nephritis-prone MRL/lpr mice. Methods：24 MRL/lpr mice （20 weeks old ）and 24 C57BL mice were equally divided into 2 groups randomly: control group (saline ,NS group) and treatment group (arsenic trioxide 0.4 mg/kg·d, ATO group) . These drugs were given by abdominal injection for 60 days.The expression of DNMT1 and CD11a was determined by RT-PCR. Results： The gray scale value of DNMT1 - mRNA in ATO group was significantly higher than that in NS group（P＜0.05）in MRL/lpr mice ,and the gray scale value of CD11a-mRNA in ATO group was significantly lower than that in NS group （P＜0.05）in MRL/lpr mice .Conclusion：The mechanism of ATO of the treatment of SLE may be related with reversed low methylation. 　　 Key words:Arsenic Trioxide Systemic Lupus;Erythematosus;DNMT1;CD11a 　　________________________________________ 　　收稿日期：2011-07-05 　　基金项目：温州市科技计划项目 ( 　　作者简介：王红（1978-），女，浙江绍兴人