简青霉对酚类和苯胺类污染物降解研究.docVIP

简青霉对酚类和苯胺类污染物降解研究 　　摘 要：在培养4 d的简青霉培养体系中,分别加入不同浓度的酚类和苯胺类物质,观察这些有毒物质对简青霉生长的影响,并研究简青霉对这几种有毒物质的降解效果.结果表明:低浓度的酚类和苯胺类物质能促进简青霉的生长,高浓度的这些物质则对简青霉的生长有一定的抑制作用.同时简青霉能够很好地催化氧化苯酚,2,4二氯苯酚和苯胺.当苯酚质量浓度为500 μg/mL，2,4二氯苯酚质量浓度为50 μg/mL，苯胺的质量浓度为1 000 μg/mL时,简青霉对这三者的降解率都接近100%.在2,4二氯苯酚质量浓度为60~125 μg/mL，苯胺质量浓度为2 000~5 000 μg/mL时,简青霉对它们的降解率分别达到60%和70%以上.简青霉对对硝基苯酚和对硝基苯胺降解效果不明显,在研究的浓度范围内,简青霉对它们的降解率只在10%左右. 　　 关键词： 苯胺类化合物;简青霉;酚类化合物;生物降解 　　 中图分类号：Q89 文献标识码：A 　　Research on the Degradation Effect of Contaminants like Phenols and Anilines by Using ??Penicillium simplicissimum ?? 　　 HU Tianjue, WU Juanjuan, ZENG Guangming, LIU Hui, ZHANG Ying, HUANG Danlian, YU Bing, SHEN Ying 　　(1.College of Environmental Science and Engineering, Hunan Univ, Changsha，Hunan 410082, China; 2.Key Laboratory of 　　 Environmental Biology and Pollution Control（Hunan Univ), Ministry of Education, Changsha,Hunan 410082,China) 　　Abstract:In order to study the growth of?? Penicillium simplicissimum ??exposed to phenols and anilines, and the degradation of these toxic substances, several different concentrations of phenols and anilines were added to the culture system, which was cultured for 4 days with Penicillium ??simplicissimum. The results have shown that low concentrations of phenols and anilines can promote the growth of ??Penicillium simplicissimum,?? But on the contrary, the growth would be restrained under the culture of high concentrations. Meanwhile, ??Penicillium simplicissimum ??can catalyze and oxidize phenol, 2,4dichlorophenol and aniline effectively. When the concentration of phenol was controlled at 500μg/mL, 2,4dichlorophenol was 50μg/mL, and aniline was 1 000μg/mL, the degradation rates of phenol, 2,4dichlorophenol and aniline were all close to 100%. When the concentration of 2,4dichlorophenol was controlled at 60~125μg/mL and aniline was 2 000~5 000μg/mL, the degradation rates were over 60% and 70%, respectively. However, the degradation effect of pnitrophenol and pnitroaniline by ??Penicillium s