葡萄叶提取物对黄瓜幼苗生长及生理特性影响.docVIP

葡萄叶提取物对黄瓜幼苗生长及生理特性影响 　　摘要采用不同浓度的葡萄叶提取物对4叶1心的黄瓜幼苗进行处理,研究了提取物对黄瓜幼苗生长及根系活力、叶绿素含量、SOD、POD和CAT活性的影响。结果表明:随着葡萄叶提取物浓度的增加,对黄瓜幼苗生长的抑制作用和黄瓜幼苗叶绿素含量均不断增加。提取物浓度为5 mg/mL时黄瓜幼苗根系活力最强,随浓度的增加根系活力不断降低。黄瓜幼苗体内的保护酶活性随浓度的增加呈先升高后下降的趋势,并在提取物浓度为10 mg/mL时,SOD和POD的活性达到峰值;提取物浓度为20 mg/mL时,CAT的活性达到峰值。 　　关键词葡萄叶提取物;黄瓜;幼苗生长;生理生化特性;影响 　　中图分类号S642.2文献标识码A文章编号 1007-5739(2010)21-0111-03 　　 　　EffectsoftheExtractsfromVitisamurensisLeavesonGrowthandPhysiologicalCharacteristicsofCucumberSeedlings 　　YUAN Qian 1,2ZENG Xu 1 *LI Lan 1YE Yu-long 1XIONG Hong-bing 1CHEN Xu-sheng 1 　　(1 Fruit Vegetable Tea Flower Institute,Yibin Academy of Agricultural Sciences in Sichuan Province,Yibin Sichuan644000; 　　2 College of Horticulture,Shenyang Agricultural University) 　　AbstractThrough different leaf extracts concentrations of Vitis amurensis treating seedlings,the effect of leaf extracts treatment on the growth,root vigor,chlorophyll content and SOD,POD,CAT activities of cucumber seedlings was studied. The results indicated that with the increasing of the extracts concentrations,the inhibition on the growth of seedlings and the contents of chlorophyll increased. The root vigor was the highest at the concentra-tion of 5 mg/mL,and then the root vigor decreased. The defense enzyme activities increased and decreased gradually with the increasing of the extracts concentrations,SOD and POD activities were the highest at the concentration of 10 mg/mL,CAT activity was the hightest at the concertration of 20 mg/mL. 　　Key wordsextracts from Vitis amurensis leaves;cucumber;seedlings growth;physiological characteristics;effect 　　 　　葡萄科(Vitaceae)植物含有大量抗微生物活性的化学成分,如黄酮类、萜类、酚酸类、生物碱、甾醇类、挥发油、芳香化合物等。医学方面对此研究较多,其水浸液对某些真菌产生不同程度抑制作用的特点,已被应用于药理作用的抗炎研究中[1-2]。中国葡萄科植物资源丰富,全世界共12个属,中国有7个属,且多为药用植物,因此具有很大的研究价值[3]。周璇等[4]对蔬菜、花卉、树木、作物和杂草中的29科73种植物提取物抑菌活性进行了筛选,发现葡萄叶提取物有很强的抑菌活性。近年来关于植物源提取液防治病害的报道多集中于其对病原菌的抑制作用,而关于植物源提取液改变植物体自身抗性从而抑制病原菌侵害的研究报道较少。本研究旨在探索葡萄叶提取物对黄瓜幼苗的生长、根系活力、叶绿素含量和体内保护酶活性的影响,进一步了解提取物对黄瓜自身的影响,为解决黄瓜生理抗性问题提供科学依据。 　　1材料与方法 　　1.1供