芬太尼联合丙泊酚对大鼠肺缺血再灌注损伤保护作用.docVIP

芬太尼联合丙泊酚对大鼠肺缺血再灌注损伤保护作用 　　[摘要] 目的 探讨芬太尼联合丙泊酚作用于大鼠肺缺血再灌注损伤(I／R)模型后，对核因子-κB（nuclear factor-κB）表达的影响及其机制。方法 将40只雄性Wistar大鼠随机分为5组（n=8）：假手术组（S组）、缺血再灌注损伤组（I/R组）、芬太尼干预组（F 组）,丙泊酚干预组（P 组），芬太尼联合丙泊酚干预组（F+P组）。对大鼠动脉血进行血气分析；检测大鼠肺组织湿/干（W/D）值；对大鼠肺组织进行大体形态学及HE染色后镜下病理学观察，采用免疫组化法测定大鼠肺组织NF-κB表达水平的变化。 结果 与I/R组比较，F组、P组以及F+P组大鼠肺组织核因子-κB表达明显降低，差异有统计学意义（P0.01）；大鼠动脉血PaO2值升高，差异有统计学意义（P＜0.01）；大鼠肺组织W/D值明显降低，差异有统计学意义（P＜0.01）。I/R组肺毛细血管扩张充血，肺泡间质水肿并有炎性细胞浸润，肺泡腔内可见炎性细胞渗出。假手术组（S组）、芬太尼干预组(F 组),丙泊酚干预组（P 组），芬太尼联合丙泊酚干预组(F+P组)在肺泡结构较完整性，减轻肺间质及肺泡腔渗出、水肿肺组织保护方面均较I/R组明显减轻。 结论 芬太尼联合丙泊酚干预可显著减轻大鼠肺缺血再灌注损伤，可能是芬太尼联合丙泊酚能降低大鼠肺组织NF-κB水平有关。 　　[关键词] 芬太尼；丙泊酚；核因子-κB；缺血再灌注损伤 　　[中图分类号] R614[文献标识码] A[文章编号] 1673-9701（2014）15-0010-04 　　 　　Protective effects of fentanyl combined with propofol on pulmonary ischemia reperfusion injury in rats 　　WEN Wen GUO Guangwei HUANG Zhigang SUN Guodong LI Xiaohui 　　Department of Cardiothoracic Surgery，the Second Affiliated Hospital of Shanxi Medical University，Taiyuan 030001，China 　　[Abstract]Objective To study the expression of nuclear factor-κB(NF-κB) in the lung and plasma separately and explore the possible mechanisms of fentanyl combine with propofol on lung ischemia-reperfusion injury of rats. Methods The 40 Wistar male rats weighing 220 ～ 270 g were divided randomly into five groups which were pretreated differently. The values of NF-κB in rats’ lung was detected by using immunohistochemical method, the lung tissue observed in vitro and under the microscope after HE staining， the rats’ arterial oxygen pressure and the lung tissue W / D ratio were measured in each group. Results Compared with the I / R group, the F group, the P group, and the F+P group all followed the same trend: the NF-κB expression levels were obviously lower (P＜0.01), the values of PaO2 were obviously higher(P＜0.01), and the W / D ratios were lower(P＜0.01). Conclusion Fentanyl combined with propofol can significantly reduce lung ischemia-reperfusion injury, it may be that fentanyl combined with propofol can