艾滋病热毒蕴结证与脾肾阳虚证差异蛋白质表达的 研究-中医临床基础专业论文.docxVIP

万方数据 万方数据 摘要 目的 通过比较艾滋病期患者热毒蕴结证和脾肾阳虚证间的差异表达蛋白质，探索 艾滋病期患者热毒蕴结证和脾肾阳虚证辨证分型的分子标志物，为艾滋病的中医 证候诊断研究提供客观依据。 方法 采集艾滋病期热毒蕴结证、脾肾阳虚证患者和健康对照组外周血，提取蛋白 质，经过蛋白质消化、iTRAQ 标记、质谱分析，筛选并鉴定出差异表达蛋白质， 对差异表达蛋白质进行生物信息学分析。年龄、性别等计数或计量资料采用 SPSS19.0 统计软件分析。 结果 艾滋病热毒蕴结证和脾肾阳虚证两证型间获得差异表达在 1.2 倍以上的差异 蛋白质 141 个，他们主要涉及肽链内切酶活性、抗原结合、脂质结合、丝氨酸内 肽酶活性、丝氨酸水解酶活性等分子功能和免疫反应、免疫反应的调控、组织稳 态、防御反应、内稳定过程、应激反应、应激反应的调控、对刺激反应的正调节 等生物学过程。其中功能显著的差异蛋白质 5 个，分别是 GSH-Px、KRT1、C5、 Apo D、Apo A-IV。 结论 1、艾滋病热毒蕴结证和脾肾阳虚证两个证型间存在蛋白质差异表达谱，蛋 白质组学研究方法有望为中医辨证的准确性提供客观的参考依据。 2、本研究结果显示 GSH-Px、KRT1、C5、Apo D、Apo A-IV 可能是艾滋病 两证型间特异性的差异表达蛋白质，可作为艾滋病中医辨证分型的分子标志物。 关键词 艾滋病；证候；热毒蕴结证；脾肾阳虚证；蛋白质组学 2 Abstract Objective This study was designed to explore the molecular markers in the syndrome differentiation of AIDS and provide objective basis to the TCM syndrome research of AIDS by comparing the different expressed proteins between AIDS with Spleen-Kidney Yang Deficiency Syndrome and Heat-toxin Stagnation Syndrome. Methods Protein was extracted from peripheral blood samples of the AIDS patients with Spleen-Kidney Yang Deficiency Syndrome,Heat-toxin Stagnation Syndrome and healthy volunteers.We screened ,identified the different expressed proteins by protein digestion, iTRAQ labeling and mass spectrometry analysis. The different expressed proteins were analyzed with relative bioinformatics methods. SPSS19.0 statistical software was used to analyze the enumeration data and measurement data , like age , sexuality and so on. Results One hundred and forty-one differential expression proteins were obtained between AIDS with Spleen-Kidney Yang Deficiency Syndrome and Heat-toxin Stagnation Syndrome，which mainly related to the molecular functions of endopeptidase activity、 antigen binding、lipid binding、serine-type endopeptidase activity、serine hydrolase activity 、 etc ， and mainly on many biological processes including immune response,regulation of immune response 、 tissue homeostasis 、 defense response 、 homeostatic process、response to stress、positive r