莪术醋制对大鼠胆汁代谢影响.docVIP

莪术醋制对大鼠胆汁代谢影响 　　[摘要]通过考察莪术醋制前后对由肝脏分泌的胆汁中内源性代谢产物的影响，以期探讨莪术醋制对胆汁内源性代谢物的影响。分别制备生、醋莪术醇提液及生理盐水溶液，灌胃给药05 h后做胆总管插管引流术收集12 h内大鼠胆汁；胆汁样品1∶3乙腈沉淀蛋白后进行UPLCTOFMS分析；采用单维统计与多元统计相结合，PeakView软件与网络数据库相比对的数据分析方法，鉴定潜在生物标记物。 醋制前后莪术提取物对大鼠胆汁代谢谱具显著影响，鉴别出生、醋莪术给药组的大鼠胆汁中具有显著性差异的13个代谢物；再结合化合物网络数据库进行鉴定，得到8个代谢物；进一步把给药组与空白组进行t检验单维统计分析，得到与空白组比较具显著性差异的7个代谢物，推定此7个代谢物为生、醋莪术给药后大鼠胆汁的潜在生物标记物，其中6个潜在生物标记物在醋品组中显示上调，其与磷脂代谢、脂肪代谢、胆汁酸代谢及与调控N酰基乙醇胺水解反应平衡作用相关的代谢调控相关，提示通过大鼠胆汁内源性代谢物的差异探讨莪术醋制增效的机制。 　　[关键词]莪术；醋制；胆汁；代谢组学；UPLCTOFMS 　　[Abstract]To explore the effect of vinegarprocessed Curcumae Rhizoma on endogenous metabolites in bile by investigating the endogenous metabolites difference in bile before and after Curcumae Rhizoma was processed with vinegar Alcohol extracts of crude and vinegarprocessed Curcumae Rhizoma， as well as normal saline were prepared respectively， which were then given to the rats by intragastric administration for 05 h Then common bile duct intubation drainage was conducted to collect 12 h bile of the rats UPLCTOFMS analysis of bile samples was applied after 1∶3 acetonitrile protein precipitation； unidimensional statistics were combined with multivariate statistics and PeakView software was compared with network database to identify the potential biomarkers Vinegarprocessed Curcumae Rhizoma extracts had significant effects on metabolites spectrum in bile of the rats With the boundaries of P005， 13 metabolites with significant differences were found in bile of crude and vinegarprocessed Curcumae Rhizoma groups， and 8 of them were identified when considering the network database Ttest unidimensional statistical analysis was applied between administration groups and blank group to obtain 7 metabolites with significant differences and identify them as potential biomarkers 6 of the potential biomarkers were upregulated in vinegarprocessed group， which were related to the metabolism regulation of phospholipid metabolism， fat metabolism， bile acid metabolism， and Nacylethanolamine hydrolysis react