胡柚皮黄酮对高脂血症大鼠降血脂作用研究.docVIP

胡柚皮黄酮对高脂血症大鼠降血脂作用研究 　　[摘要]观察胡柚皮黄酮（PTFC）对高脂血症大鼠的降血脂作用，初步探索其作用机制。以高脂饮食4周建立高脂血?Y大鼠模型，造模同时分别予以50，100，200 mg?kg-1?d-1的PTFC灌胃干预4周。造模2周末取尾血，生化法检测血清TC，TG，HDL-C水平，并称量体重。造模4周末，称量大鼠体重及其肝脏质量，检测血清TC，TG，HDL-C，LDL-C，ALT，AST，MDA，SOD含量，计算血脂综合指数（LDL-C/HDL-C，LDL-C/TC）及动脉硬化指数（AI），生化法检测肝组织MDA，SOD水平。HE染色观察肝组织病理学变化，ELISA法检测肝组织PPAR-α，Lpl，Lipc蛋白表达，Real-time PCR法检测肝组织PPAR-α mRNA表达。研究发现，PTFC对高脂血症大鼠体重增高有降低趋势，对大鼠肝脏质量和肝指数升高均明显降低；对血清TC，TG，LDL-C，LDL-C/HDL-C与AI升高具有明显降低作用，而对血清LDL-C/TC降低有明显升高作用；PTFC干预后，能明显减轻高脂血症大鼠组织脂肪变性和炎症程度，显著改善肝功能；显著改善高脂血症大鼠体内氧化应激水平。同时能显著促进高脂血症大鼠肝组织PPAR-α，Lpl，Lipc的蛋白表达和PPAR-α mRNA的表达。研究表明PTFC通过有效地积极调控与脂肪分解代谢有关的PPAR-α的基因表达及PPAR-α，Lpl，Lipc的蛋白表达，并提高机体的抗氧化能力，降低氧化应激水平，实现对脂代谢的积极调控。 　　[关键词] 胡柚皮黄酮； 高脂血症； 氧化应激； 过氧化物酶体增殖体活化受体α（PPAR-α）； 脂蛋白酶（Lpl）； 肝脂酶（Lipc） 　　[Abstract] To observe and investigate the effects and mechanisms of the pure total flavonoids from Citrus changshan-huyou（PTFC） on blood lipid metabolism in hyperlipidemic rats. SD rats were fed with high fat diet for 4 weeks to induce hyperlipidemic rats model， meanwhile three dosages （50， 100， 200 mg?kg-1?d-1） of PTFC were administrated intragastrically for 4 weeks respectively.After 2 weeks of modeling， their tail blood was taken and serum TC， TG， and HDL-C levels were detected by biochemical method and their body weight was measured. After 4 weeks of modeling， their body weight was measured and liver weight was measured， then the levels of TC， TG， HDL-C， LDL-C， ALT， AST， MDA and SOD in serum were detected to calculate lipid comprehensive index（LDL-C/HDL-C and LDL-C/TC ratios） and atherogenic index（AI）； in addition， MDA and SOD levels were detected by biochemical method. The hitopathological changes of the liver tissues were observed by HE staining； the protein expression levels of PPAR-α， Lpl， and Lipc were detected by ELISA； and the mRNA expression levels of PPAR-α in the liver tissue were detected by Real-time PCR. The results showed that gavage administration of the PTFC significantly decreased the body weight， liver weight， l