脑胶质瘤患者脑脊液中HOXA13Plexin―B2定量分析及生物学意义研究.docVIP

脑胶质瘤患者脑脊液中HOXA13Plexin―B2定量分析及生物学意义研究 　　[摘要] 目的 探讨同源基因A-13（homeotic genes-A13，HOXA13）和神经丛素-B2（Plexin-B2）在脑胶质瘤中的表达情况及生物学意义。 方法 收集2014 年5月～2016年2月间我院诊断的脑胶质瘤患者60例，使用 qRT-PCR技术分别检测实验组（脑胶质瘤患者）和对照组（健康受试者）血清中S100、HOXA13和Plexin-B2的表达水平，并分析HOXA13和Plexin-B2在不同级别脑胶质瘤患者中的表达情况，分析其与肿瘤恶性程度的关系。 结果 与对照组对比，实验组的HOXA13和Plexin-B2在脑脊液及血浆中表达水平显著升高[血液中：HOXA13，（1.21±0.14） vs 0；Plexin-B2：（1.57±0.33） vs 0；脑脊液中：Plexin-B2：（4.87±1.13） vs 0；Plexin-B2：（5.88±0.21） vs 0]，肿瘤体积越大、分化程度越低，血液中HOXA13和Plexin-B2表达水平越高；脑脊液中HOXA13和Plexin-B2蛋白水平明显高于血浆中。 结论 脑胶质瘤患者中HOXA13和Plexin-B2表达上调，且与肿瘤恶性程度有显著关系，脑脊液中HOXA13和Plexin-B2表达显著升高，可用作脑胶质瘤恶性程度的标志物。 　　[关键词] 脑胶质瘤；同源基因A-13；神经丛素-B2；qRT-PCR 　　[中图分类号] R739.41 [文献标识码] A [文章编号] 1673-9701（2017）15-0023-04 　　[Abstract] Objective To investigate the expression and biological significance of gomeotic genes-A13（HOXA13） and neuronal-B2（Plexin-B2） in gliomas. Methods 60 patients diagnosed with glioma were diagnosed in our hospital from May 2014 to February 2016 were chosen. The serum expression levels of S100， HOXA13 and Plexin-B2 in the experimental group（glioma patients） and the control group（health volunteers） were measured by qRT-PCR. And the expression of HOXA13 and Plexin-B2 in different stages of glioma patients was analyzed， the relationship with the degree of tumor malignancy was analyzed. Results Compared with those of the control group， the expression levels of HOXA13 and Plexin-B2 in cerebrospinal fluid and plasma were significantly higher[blood： HOXA，13，（1.21±0.14） vs 0； Plexin-B2， （1.57±0.33） vs 0； cerebrospinal fluid： Plexin-B2， （4.87±1.13） vs 0； Plexin-B2， （5.88±0.21） vs 0]. The larger the tumor volume， the lower the degree of differentiation and the higher the expression level of HOXA13 and Plexin-B2 in the blood. The levels of HOXA13 and Plexin-B2 protein in cerebrospinal fluid were significantly higher than those in plasma. Conclusion The expression of HOXA13 and Plexin-B2 in brain glioma patients is up-regulated and is significantly correlated with the degree