壁虎醇提物抗肿瘤活性的初步分析-药理学专业论文.docxVIP

摘要 摘要 tumor inhibitory rates were 44.88%, 63.94% and 69.53%, respectively. However, the levels of serum TNF-a of mice were no change. The tumor inhibitory rates of intravenous administration of GAE(0.6g/kg, 1.2g/kg, 2.4g/kg) combined with CTX (20mg/kg) were 56.93%, 67.15％and 70.24%, which were higher than that of CTX administration alone (41.71％). Compared with those in CTX group, the count of WBC (P0.01), the index of thymus and spleen (P0.05) were significantly elevated in all GAE and CTX combination groups. CONCLUSION: In tumor inhibitory test, it is shown that GAE may possess significantly inhibitory effect in vitro and in vivo. Antitumor mechanism of GAE on EC9706 cells can induce cell apoptosis which may be associated with the increased Bax/Bcl-2 ratio in EC9706 cells. GAE has synergism and attenuation effects on CTX. KEY WORDS: Gecko alcohol extract, human esophageal squamous carcinoma cell line EC9706, apoptosis protein, S180-bearing mice, synergism and attenuation, TNF-a Dissertation Type: Fundamental Study IV 缩略语词汇表 缩略语词汇表 缩略语词汇表 缩略词 英文全称 中文全称 GAE Gecko alcohol extract 壁虎醇提物 CTX Cytoxan 环磷酰胺 PBS phosphate buffered saline 磷酸盐缓冲液 DMSO dimethyl sulfoxide 二甲基亚砜 OD optical density 光密度 TUNEL TdT-mediated dUTP TdT 介导的 dUTP 缺 nick end labeling 口末端标记技术 AR Apoptotic Rate 凋亡率 S180 sarcoma 180 小鼠肉瘤细胞株 TNF-a tumor necrosis factor-alpha 肿瘤坏死因子-a ELISA enzyme-linked immunosorbent assay 酶联免疫吸附法 HE hematoxylin eosin 苏木素-伊红染色 MTT methylthiazolyltetrazolium 四氮唑兰 HRP horse-radish peroxi