血管内皮生长因子和雌二醇促进血管内皮祖细胞分化生成血管对比研究.docVIP

血管内皮生长因子和雌二醇促进血管内皮祖细胞分化生成血管对比研究 　　[摘要]目的：对比研究常规剂量下血管内皮生长因子（VEGF）和雌二醇对血管内皮祖细胞（EPCs）分化生成血管的促进作用。方法：分离培养人外周血EPCs，与基质胶混匀后注射到9只裸鼠双侧下腹部，另设2只注射等体积培养液与基质胶的混合液。将9只注射细胞的裸鼠随机分为3组，每组分别定期局部注射VEGF、雌二醇，生理盐水，定期观察记录血管组织块的生长状况。移植6周后取材，测量计算血管组织块的体积、HE染色观察血管组织块的血管增殖状况，各组之间进行对比。结果：给药组血管组织块体积与注射生理盐水组相比，具有显著性差异，体积明显偏大，而给药组之间体积未见显著性差异。HE染色观察可见各组的血管组织块内血管增殖明显，血管排列紊乱的，管腔大小不一。给药组血管密度明显大于注射生理盐水组，而给药组之间的血管密度差异较小。结论：VEGF和雌二醇组具有促进EPCs分裂增殖形成血管的能力，常规剂量下两者促进EPCs分裂增殖生成血管的能力无显著性差异。 　　[关键词]内皮祖细胞；血管；血管内皮生长因子；雌二醇。 　　[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2013）01-0048-03 　　Comparative study of vascular endothelial growth factor and estradiol in promoting endothelial progenitor cells differentiation and generation blood vessels 　　DONG Yong，LI Wen-zhi，XIN Yi，SUN Zhi 　　（Department of Plastic Surgery and Laser Medicine，Beijing Anzhen Hospital of Capital Medical University Beijing Institute of Cardiopulmonary and Vascular Disease，Beijing 100029，China） 　　Abstract： Objective To compare the ability of vascular endothelial growth factor （VEGF） and estradiol in promoting endothelial progenitor cells differentiation and generation blood vessels under common doses. Methods To isolate and culture human peripheral blood EPCs first， then to mixe with the matrigel and transplante to the lower abdomens of nine nude mice，to divide the nine nude mice into three groups according to a random grouping， to inject VEGF，estradiol and saline at a regular time，to observe and record the growing status of vascular tissue regularly，to draw the vascular tissue after six weeks，to observe the organizational structure by HE staining， then contrast with the groups. Results The vascular tissue volume groups injected of drugs have significant difference with the groups injected of saline，they have bigger volume to contrast the groups injected of saline， but he vascular tissue volume between the groups injected of drugs is no significant difference. By drawning HE staining， the vascular tissues have proliferational mussy blood vessels