藏药波棱瓜子总木脂素对四氯化碳致大鼠肝纤维化保护作用及机制探讨.docVIP

藏药波棱瓜子总木脂素对四氯化碳致大鼠肝纤维化保护作用及机制探讨 　　[摘要]?实验探讨藏药波棱瓜子总木脂素对四氯化碳（CCl4）致大鼠肝纤维化的保护作用及其作用机制。将SD大鼠48只随机分为空白对照组、模型组、总木脂素低、中、高剂量（100，200，400 mg?kg-1）组、阳性药甘利欣（25 mg?kg-1）组，每组8只。空白组正常饲养，模型组、总木脂素低、中、高剂量组以及甘利欣组大鼠均隔2～3 d皮下注射3 mL?kg-1含40%CCl4的橄榄油溶液连续造模8周。期间空白组、模型组灌胃等量生理盐水2 mL，总木脂素低、中、高剂量组、甘利欣组灌胃给药相应剂量。8周后最后一次造模6 d后处死大鼠。生化分析法测定各组大鼠血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）含量。ELISA法检测血清肝纤维化指标转化生长因子-β1（TGF-β1）、透明质酸（HA）、羟脯氨酸（HYP）、超氧化物歧化酶（SOD）的水平，肝组织进行HE和Masson胶原染色，进行病理分析并评分。结果显示与模型组大鼠比较，总木脂素组大鼠血清中ALT，AST，ALP，TGF-β1，HA和HYP水平显著降低（P1表达有关。 　　[关键词]总木脂素； 肝纤维化； TGF-β1 　　[Abstract]This experiment was aimed to discuss the protective effect and mechanism of total lignans from Tibetan medicinal Herpetospermum seeds on carbon tetrachloride （CCl4）-induced liver fibrosis in rats. Forty-eight male Sprague-Dawley rats were randomly divided into the blank control group （G1）， model group （G2）， total lignans high， middle and low dose groups （400，200， and 100 mg?kg-1?d-1）（G3/4/5） and the glycyrrhizin positive control group （25 mg?kg-1?d-1）（G6）， N=8 in each group. The rats in blank group received normal feeding； the rats in model group， total lignans low， middle and high dose groups and glycyrrhizic group were subcutaneously in jected with 3 mL?kg-1 olive oilsolution containing 40%CCl4every two or three days for Eight weeks. During the course， the rats inblank group and model group were orally administered with 2 mL normal saline， and the rats in total lignans groups and the glycyrrhizin positive control group received corresponding doses of drugs by intragastric administration. Eight weeks later， after the the last time modeling， the rats were sacrificed. Then the biochemical analysis was used to determine alanine aminotransferase（ALT）， aspartate aminotransferase（AST）， and alkaline phosphatase （ALP） levels in serum while enzyme-linked immuno sorbent assay（ELISA） was applied for detecting transforming growth factor β1（TGF-β1）， hyaluronic acid（HA）， hydroxy-proline（HYP） and superoxide dismutase（SOD） levels in seru