藏药镰形棘豆鼠李柠檬素对免疫抑制小鼠非特异性免疫功能影响.docVIP

藏药镰形棘豆鼠李柠檬素对免疫抑制小鼠非特异性免疫功能影响 　　摘要：目的 探讨藏药镰形棘豆提取物鼠李柠檬素增强免疫的作用机制。方法 选用昆明种小鼠，随机分为空白组、模型组、阳性药组及中药高、中、低剂量组，各给药组给予相应药物灌胃，连续10 d。第8日起，除空白组腹腔注射生理盐水外，其他各组均腹腔注射环磷酰胺，连续3 d。通过巨噬细胞炭粒廓清试验、巨噬细胞吞噬鸡红细胞试验、免疫抑制小鼠器官指数测定，观察鼠李柠檬素对免疫抑制小鼠非特异性免疫功能的影响。结果 与模型组和空白组比较，中药各剂量组能提高免疫抑制小鼠的吞噬活性、廓清指数和吞噬系数（P0.05），增加免疫器官脏器指数；与阳性药组比较，中药各剂量组药效较低。结论 藏药镰形棘豆鼠李柠檬素具有一定的免疫增强作用，可促进小鼠鸡红细胞免疫后脾细胞血清溶血素的形成。 　　关键词：镰形棘豆；鼠李柠檬素；免疫抑制；非特异性免疫；小鼠 　　DOI：10.3969/j.issn.1005-5304.2015.05.016 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2015）05-0057-03 　　Abstract：Objective To study action mechanism of rhamnocitrin in Oxytropis Falcata on enhance the immune function. Methods Kunming mice were randomly divided into blank control group， model group， positive control group， traditional Chinese medicine high， medium， and low dose groups. All treatment groups received gavage with relevant medicine for 10 days. From the 8th day， blank control group received intraperitoneal injection with normal saline， while other groups received intraperitoneal injection with cyclophosphamide for 3 days. Through macrophages carbon clearance test， macrophage phagocytosis of chicken erythrocytes test， and immunosuppressive mice organ index detection， effects of rhamnocitrin in Oxytropis Falcata on nonspecific immune function of immunosuppressive mice were observed. Results Compared with model group and blank control group， traditional Chinese medicine high， medium， and low dose groups could improve the phagocytic activity of immunosuppressive mice， clearance index， and phagocytic coefficient （P0.05）， and increase the organ index of the thymus and spleen. Compared with the positive control group， the efficacy of traditional Chinese medicine high， medium， and low dose groups was low. Conclusion Rhamnocitrin in Oxytropis Falcata can enhance the immune function， improve the formation of spleen cells of mice serum hemolysin of chicken red blood cell immune. 　　Key words：Oxytropis Falcata；rhamnocitrin；immunosuppression；nonspecific immunity；mice 　　镰形棘豆来源于藏药豆科植