电针预处理对小鼠全脑缺血再灌注ampk表达的影响-麻醉学专业论文.docxVIP

万方数据 Effect of electroacupuncture preconditioning on expression of AMPK in hippocampus following transient cerebral ischemia-reperfusion in mice Abstract Objective: To investigate the effect of electroacupuncture preconditioning on expression of AMPK and apoptosis of neurocytes in hippocampus of mice following transient cerebral ischemia-reperfusion. Methods: Sixty healthy male C57BL/6 mice were randomly assigned into 5 groups: group Sham, BCCAO, EA+BCCAO, CC+BCCAO and EA+CC+BCCAO. Sham group only subjected to cervical median incision served as blank control and BCCAO group underwent 15 minutes bilateral common carotid artery occlusion (BCCAO) without any other treatment. EA+BCCAO group was continuously administered electroacupuncture preconditioning before BCCAO and CC+BCCAO group was given Compound C (AMPK inhibitor) before BCCAO. EA+CC+BCCAO group was continuously administered electroacupuncture preconditioning and then given Compound C before BCCAO. Seventy-two hours after reperfusion, all mice were harvested and the expression level of AMPK and p-AMPKα in brain was investigated by Western blot. The morphological alteration and number of neurocytes surviving in hippocampus CA1 was measured by HE staining and TUNEL. Results: In contrast with BCCAO group, the proportion of apoptosis neurocytes in EA+BCCAO group decreased significantly and the expression of AMPKα increased significantly (P ＜ 0.05). Compared to EA+CC+BCCAO group, the proportions of neurocytes surviving in EA+BCCAO group increased significantly while expression of p-AMPKα increased significantly (P＜0.05). Conclusion: Electroacupuncture preconditioning could protect brain from ischemia-reperfusion injury by up-regulating the AMPKα and p-AMPKα expression. Postgraduate student: Ran Qiangqiang(Anesthesiology) Directed by Prof: Wang Mingshan Key words: Electroacupuncture preconditioning; AMPK; cerebral protection; ischemia-reperfusion injury  目 录 引言 1 第一章 材料与方法 2 1.1 实验动物与分组： 2 1.2