重组人甲状旁腺素对人骨髓间充质干细胞表达骨形态发生蛋白2影响.docVIP

重组人甲状旁腺素对人骨髓间充质干细胞表达骨形态发生蛋白2影响 　　【摘要】 目的：探讨不同浓度的重组人甲状旁腺素（hPTH1-34）对人骨髓间充质干细胞骨形态发生蛋白2（bone morphogenetic protein 2，BMP2）表达的影响。方法：培养人骨髓间充质干细胞，分为空白对照组、阳性对照组、hPTH1-34组（共3组，PTH浓度分别为40 μg/L、400 μg/L、4000 μg/L组）。给药后第4、7、14天检测碱性磷酸酶活性、BMP2的表达。结果：给药第7、14天，较高剂量组（4000 μg/L、400 μg/L）碱性磷酸酶活性较低剂量组（40 μg/L）有下降。给药第14天，所有给药组的碱性磷酸酶活性均低于阳性对照组（P0.05）。给药第7、14天，较高剂量组（4000 μg/L、400 μg/L）的BMP2含量低于低剂量组（40 μg/L）（P0.05）。hPTH1-34低剂量组（40 μg/L）BMP2浓度较空白对照组明显高（P0.05）。结论：在一定浓度范围内，hPTH1-34间歇用药有促成骨细胞增生与分化作用，并呈时间依赖性；超过一定浓度后则反而抑制成骨细胞的增殖与分化。 　　【关键词】 重组人甲状旁腺素； 人骨髓间充质干细胞； BMP2 　　中图分类号 R329.2 文献标识码 A 文章编号 1674-6805（2016）4-0005-03 　　Effect of Recombinant Human Parathyroid Hormone on the Expression of Bone Morphogenetic Protein 2 in Human Bone Marrow Mesenchymal Stem Cells/LI Ding，WANG Jun-fang，ZHANG Cheng-yu.//Chinese and Foreign Medical Research，2016，14（4）：5-6 　　【Abstract】 Objective：To detect the effect of recombinant human parathyroid hormone（hPTH1-34） on the expression of bone morphogenetic protein 2（BMP2） in human bone marrow mesenchymal stem cells.Method：The cultured human bone marrow mesenchymal stem cells（MSCs） were divided into blank control group，positive control group，hPTH1-34 group（the concentrations were 40 μg/L，400 μg/L，4000 μg/L group）.The alkaline phosphatase activity and the concentration of BMP2 were detected in the 4th，7th and 14th day after administration.Result：In the 7th and 14th day，the alkaline phosphatase activity of high dose group（4000 μg/L，400 μg/L） were lower than low dose group（40 μg/L）.In the 14th day，the activities of the alkaline phosphatase in all hPTH1-34 groups were lower than the positive control group.In the 7th and 14th day，the BMP2 concentration in high dose group（4000 μg/L，400 μg/L） were lower than low dose group（40 μg/L）.The BMP2 concentration in low dose group（40 μg/L） was significantly higher than the control group.Conclusion：In a certain concentration range，intermittent medication of hPTH1-34 can promote the proliferation and differentiation of osteoblast in