阿托伐他汀对强直性脊柱炎患者外周血单个核细胞体外干预后Toll样受体2Toll样受体4表达变化研究.docVIP

阿托伐他汀对强直性脊柱炎患者外周血单个核细胞体外干预后Toll样受体2Toll样受体4表达变化研究 　　[摘要] 目的：观察天然免疫分子Toll样受体2（TLR-2）、Toll样受体4（TLR-4）在强直性脊柱炎（ankylosing spondylitis，AS）患者外周血单个核细胞（peripheral blood mononuclear cells，PBMC）的mRNA水平表达，研究其与AS发病的相关性，并探讨阿托伐他汀是否对AS患者TLR-2、TLR-4的表达有影响。方法：研究对象包括AS活动期患者30例和健康志愿者30例，分别抽取外周静脉血分离单个核细胞，提取细胞mRNA。采用半定量的RT-PCR方法，检测AS患者和健康志愿者外周血单个核细胞中TLR-2、TLR-4的mRNA，通过灰度分析与β-actin相比得到目的基因相对表达水平。分离12例AS活动期患者静脉血外周血单个核细胞体外培养，并给予阿托伐他汀（终浓度为0.01 mmol/L）处理，以自身非处理细胞为对照，24 h后收获细胞，提取mRNA，采用RT-PCR方法，观察阿托伐他汀对细胞TLR-2、TLR-4的mRNA表达的影响。所有数据采用配对t检验。结果：AS患者TLR-2 mRNA的相对表达水平为（1.59±1.43），较健康志愿者（0.06±0.039）高（P0.05）；AS患者TLR-4 mRNA的相对表达水平为（0.60±0.73），较健康志愿者（0.06±0.039）高（P0.05）；阿托伐他汀干预组TLR-2和TLR-4的mRNA表达均低于不干预对照组（P0.05）。结论：AS患者PBMC的TLR-2、TLR-4的表达均较健康志愿者高，提示TLR-2和TLR-4可能参与了AS的发病。阿托伐他汀可下调AS患者PBMC的TLR-2和TLR-4的表达，可能是AS新的有价值的治疗靶点之一。 　　[关键词] 强直性脊柱炎；Toll样受体；阿托伐他汀 　　[中图分类号] R593.23 [文献标识码] A [文章编号] 1673-7210（2012）01（a）-025-03 　　 　　Expression of Toll-like receptor 2 and Toll-like receptor 4 after intervention by Atorvastatin work on peripheral blood mononuclear cells of ankylosing spondylitis patients 　　WU Jing, LIANG Wanyi, DENG Jiaxin, QU Yuan, YU Qinghong 　　Department of Rheumatology and Immunology, Zhujiang Hospital of Southern Medical University, Guangdong Province, Guangzhou 510280, China 　　[Abstract] Objective: To study the expression and regulation on TLR-2 and TLR-4 after intervention by Atorvastatin working on the peripheral blood mononuclear cells (PBMC) of ankylosing spondylitis patients. Methods: The study was designed to enroll 30 cases of AS and 30 cases of healthy controls. The expression levels of TLR-2 and TLR-4 in PBMC were determined by RT-PCR technical through comparing with β-actin, used t-test to compare the means. In vitro effect of Atorvastatin (0.01 mmol/L) working on PBMC of ankylosing spondylitis patients, 12 cases were entrolled in. PBMC from one patient were divided into two groups. One was treated with Atorvastatin (0.01 mmol/L), another was control. The different expression leve