第四脑室注射orexin-A和OX1R拮抗剂对大鼠摄食和自由活动.PDF

生理学报Acta Physiologica Sinica , August 25, 2015, 67(4): 379–385 379 DOI: 10.13294/j.aps.2015.0046 研究论文 第四脑室注射orexin-A及OX1R拮抗剂对大鼠摄食及自由活动 的影响 1 1 1 2 1,* 彭晓燕 ，郭菲菲，孙向荣，公衍玲，徐 珞 1 2 青岛大学医学院病理生理教研室，青岛 266021 ；青岛科技大学化工学院病理生理教研室，青岛 266021 摘 要：本文旨在探讨第四脑室注射orexin-A及orexin 1型受体(orexin-1 receptor, OX1R)拮抗剂SB334867对肥胖和正常大鼠摄 食和自由活动的影响。采用高脂饲料诱导建立肥胖大鼠模型，分别在肥胖和正常大鼠第四脑室注射不同剂量orexin-A或 SB334867，观察光照和黑暗环境下两种大鼠0~4 h摄食量及活动量的变化。结果显示，第四脑室注射不同剂量orexin-A ，光 照条件下，正常和肥胖大鼠0~4 h摄食量和活动量均较生理盐水对照组明显增加，呈剂量依赖关系(P 0.05~0.01) ；且肥胖大 鼠摄食量和活动量显著高于正常大鼠；黑暗条件下，不同剂量的orexin-A对正常和肥胖大鼠摄食量和活动量均没有明显改变 (P 0.05) 。第四脑室注射不同剂量SB334867，光照条件下，正常和肥胖大鼠0~2 h，2~4 h摄食量和活动量均较生理盐水对照 组明显减少(P 0.05) ；且肥胖大鼠摄食量和活动量均显著高于正常大鼠；黑暗条件下，正常和肥胖大鼠摄食量和活动量均 没有明显改变(P 0.05) 。以上结果提示，第四脑室周核团可能是orexin-A及OX1R作用靶点之一；光照条件对orexin-A和 OX1R生理功能的发挥可能具有重要影响。 关键词：orexin-A ；SB334867；摄食；自由活动；肥胖；大鼠 中图分类号：R329 Effect of orexin-A and orexin-1 receptor antagonist injected into the fourth ven- tricle of rats on food-intake and spontaneous physical activity 1 1 1 2 1,* PENG Xiao-Yan , GUO Fei-Fei , SUN Xiang-Rong , GONG Yan-Ling , XU Luo 1Department of Pathophysiology, Qingdao University Medical College, Qingdao 266021, China; 2Department of Pathophysiology, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266021, China Abstract: The present study was aimed to investigate the effects of orexin-A and orexin-1 receptor (OX1R) antagonist injected into the fourth ventricle of rats on food-intake and spontaneous physical activity (SPA). Obese rat model was induced by high fat diet. Dif- ferent doses of orexin-A or SB334867, an OX1R antagonist, were injected into the fourth ventricle of obese and normal rats respec- tively. SPA and food intake were monitored for 4 h