中国沙棘叶黄酮类成分和生态因子相关性及其生态适宜性研究.docVIP

中国沙棘叶黄酮类成分和生态因子相关性及其生态适宜性研究 　　[摘要] 为了解决野生藏药资源日益濒危问题，推动藏药野生资源保护与适宜区的发展和提供人工栽培种植新方法，该文采用HPLC对不同产地中国沙棘叶中槲皮素、山柰素、异鼠李素进行含量测定，应用偏最小二乘回归法（PLSR），分析不同产地中国沙棘叶中黄酮类化学成分与生态因子间的相关性。该文基于Maxent模型结合运用ArcGIS软件，对中国沙棘进行适宜区区划研究。分析得出不同产地间中国沙棘叶中槲皮素、山柰素、异鼠李素含量差异明显，且影响槲皮素积累的主导因子为海拔高度、1月份均降水量和8月份均降水量，影响山柰素含量积累的主导因子为海拔高度、最冷季度降水量、12月份均降水量和3月份均气温，影响异鼠李素含量积累的主导因子为8月份均降水量、1月份均降水量、最冷季度降水量和海拔高度；实验结果表明中国沙棘在我国地区分布的适宜指数为0～0.708，适宜区面积59.05万km2，占全国总面积的6.13%，较适宜区面积达55.25万km2，占全国总面积的5.73%。对中国沙棘叶黄酮类成分与生态因子的相关性及中国沙棘生态适宜性研究，该法操作简单可行、结果可靠，为藏药资源??现可持续开发利用提供了一种新思路。 　　[关键词] 藏药； 中国沙棘叶； 黄酮； 生态因子； 生态适宜性； Maxent模型 　　[Abstract] The study aims at providing a new suitable way to promote artificial cultivation， solving the problem of resources increasingly endangered wild medicine， and protecting the wild resources of Tibetan medicine. The content of quercetin，kaempferol and isorhamnetin was determined by HPLC. The correlation between flavonoids components and ecological factors was analyzed using partial least-squares regression （PLSR）. Based on Maxent model combining using ArcGIS software， suitable regionalization for H.rhamnoides subsp. sinensis was studied.The results showed that the difference of quercetin，kaempferol and isorhamnetin content in samples from different regions were obvious. The main factors effecting quercetin content accumulation were the altitude andthe average monthly precipitation in January and August. The main factors effecting kaempferol accumulation were the altitude andthe average monthly precipitation in the coldest quarter and December. The main factors effecting isorhamnetin accumulation were the average monthly precipitation in August， January and the coldest quarter.The regional distribution suitability index for H.rhamnoides subsp. sinensis was 0-0.708. The suitable area 590 500 km2， accounting for 6.13% of the total area. The preferably suitable area was 552 500 km2， accounting for 5.73% of the total area.The methods used in the study is simple and feasible， the result is reliable