台湾翅果菊有性生殖和胚胎发育形态研究.docVIP

台湾翅果菊有性生殖和胚胎发育形态研究 　　[摘要]利用切片技术对台湾翅果菊Pterocypsela formosana胚胎发育过程进行了显微观察。研究结果表明，花药4室，药壁发育为双子叶型，由表皮、药室内壁、中层和绒毡层4层细胞组成，腺质绒毡层;小孢子母细胞减数分裂为同时型，四分体主要为四面体型，成熟花粉为2-细胞型。子房下位，2心皮，1室，单胚珠，基生胎座;单珠被，倒弯生胚珠，具发达的珠被绒毡层;大孢子孢原细胞直接发育为大孢子母细胞，属薄珠心类型;大孢子细胞减数分裂形成直线形4分体，仅合点端1个发育为功能大孢子，功能大孢子经3次连续的有丝分裂形成7细胞8核的成熟雌配子体，雌配子体发育方式为蓼型。2个极核在受精前融合为次生核，合点端反足细胞短命，形成后不久即退化消失。珠孔受精，属有丝分裂前类型;初生胚乳核早于合子分裂，胚乳发育为核型，具胚乳吸器;胚胎发育为紫菀型菊苣变型，早期发达的胚柄对胚的发育具重要意义。 　　[关键词]台湾翅果菊;胚胎学;菊科;生态适应 　　Studies on sexual organs and embryological development 　　morphology of Pterocypsela formosana 　　LONG Hua1，2， MA Xue-nilt;supgt;2lt;/supgt;， HUANG Heng-yu2* 　　（1. Key Laboratory of Plant Resources Conservation and Utilization， Jishou University， College of Hunan Province， Jishou 416000， China; 　　2.Engineering Research Center for Reproducing Fine Varieties of Chinese Medicinal Plants， Yunnan 　　University of Chinese Traditional Medicine， Kunming 650500， China） 　　[Abstract]In this study， the embryological characters of Pterocypsela formosana （Asteraceae） were investigated with the traditional paraffin section methods. The anther has 4 sporangiates， the anther wall development follows the dicotyledonous type and comprises of an epidermis， endothelium， a middle layer and a single-layered tapetum， the tapetum belongs to glandular type. Meiosis of the microspore mother cells is of the simultaneous type， for the formation of mostly tetrahedral tetrad， the mature pollen grains are 2 celled. The ovary is bicarpellate， unilocular， one ovule and basal placenta， the ovule is unitegmic， tenuinucellate， inverted campylotropous and with developed endothelium， archesporial cell of megaspore differentiates immediately below the nucellar epidermis and functions as megasporocyte after development and belongs to tenuinucellate ovule type. The megasporocyte undergoes meitotic to form a liner tetrad， only one chalazal megaspore becomes the functional megaspore which forms female gametophyte including 7-celled and 8-nucleated after three successive mit