白藜芦醇苷对缺氧-复氧HK-2细胞损伤保护作用的-第三军医大学学报.DOCVIP

白藜芦醇苷对缺氧-复氧HK-2细胞损伤保护作用的研究 胡语航1，王兴勇1,何素蓉2，杨廷芳1，徐 静1，熊 燚1，周海银1，何 蓉1 (1重庆医科大学附属儿童医院ICU，重庆 400014; 2重庆市第一人民医院儿科，重庆 400010) 摘 要：目的 探讨白藜芦醇苷（Polydatinpolydatin，PD）对缺氧 - -复氧（Hypoxiahypoxia/Rere-oxygenation，H/R)肾小管上皮细胞损伤的保护作用。方法 在缺氧仓中充入95% N2、5% CO2混合气体模拟缺氧环境，将人近曲肾小管上皮细胞（HK - 2）根据不同处理分为4组：正常对照组、H/R组、PDTC组和PD处理组，除对照组外每组均予以先缺氧24 h后复氧6 h处理；MTT法检测细胞成活数，免疫组化法检测NF-κB p65蛋白表达情况，RT-PCR检测ICAM-1 mRNA转录，ELISA法检测ICAM-1蛋白表达情况。结果 PD处理组各对应时间点HK-2细胞数量明显增多于H/R组，统计学差异显著(P 0.05)。PD处理组NF-κB p65蛋白阳性表达，ICAM-1 mRNA转录及ICAM-1蛋白表达在各对应时间点均明显低于对照组，有统计学差异( P 0.05)。PD处理组与PDTC组各对应时间点文中未见检测时间点，似乎仅做一次检测？？的各项检测指标比较均无显著统计学差异(P ＞)0.05)。结论 PD对H/R诱导的HK-2细胞损伤具有一定的保护作用。 文中未见检测时间点，似乎仅做一次检测？？ 关键词： 白藜芦醇苷；缺氧-复氧；HK-2；核因子-κB；细胞间粘附分子-1 中图法分类号： 文献标识码：A Study on the pProtective effect of polydatin on hypoxia/re-oxygenation induced HK-2 cell injury HU Yu-hang1, WANG Xing-yong1，, HE Su-rong2, YANG Ting-fang1, XU Jing1, XIONG Yi1, ZHOU Hai-yin1, HE Rong1 (1Department of ICU, Childrens Children’s Hospital，, Chongqing Medical University, Chongqing 400014; 2Department of Pediatrics, the The First People’s Hospital of Chongqing, Chongqing 400010, China) Abstract： : Objective To study the protective effect of polydatin (PD) on hypoxia/re-oxygenation induced HK-2 cell injury in vitro. Methods Human renal tubular epithelial cell line HK-2 cell was used as target cell. The experiment cells were divided into 4 groups: the control group, the model group, the pyrrolidine dithiocarbamate (PDTC) PDTC group and the PD group. Except the control group, the cells in the other three groups were exposed to hypoxia for 24 h, 30 min before which PD and PDTC were respectively added to the culture media of PDTC group and PD group, then to normoxia for 6 h. Cells Cell survival was assessed by MTT, the protein expression of nuclear factor-κB (NF-κB) protein65 was measured by immunohistochemistry , the mRNA and protein expressions of ICAM-1 were measured by RT-PCR and ELISA respectively. Resul